ArticlePloS one2022
Antibacterial efficacy and possible mechanism of action of 2-hydroxyisocaproic acid (HICA).
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Antibacterial Activity of a New Modified Peptide Lep5 R8-18, Derived From Scorpion Hemiscorpius lepturus Venom.Probiotics and antimicrobial proteins · 2026Article
- Identifying a Vaginal Microbiome-Derived Selective Antibiotic Metabolite via Microbiome Pharmacology Analysis.bioRxiv : the preprint server for biology · 2025Article
- Assessment of antibiofilm and quorum quenching potencies of environmental bacteria in controlling biofilm of food spoilage bacteria.BMC research notes · 2025Article
- Effect of Light Regime onJournal of microbiology and biotechnology · 2024Article
- Pathway and Production Differences in Branched-Chain Hydroxy Acids as Bioactive Metabolites inInternational journal of molecular sciences · 2024Article
- Fermentation of Polygonati Rhizoma aqueous extract using Lactiplantibacillus plantarum under the condition of eutrophication.Archives of microbiology · 2024Article
- Bacterial Growth Modulatory Effects of Two Branched-Chain Hydroxy Acids and Their Production Level by Gut Microbiota.Journal of microbiology and biotechnology · 2024Article
- Facemask acne attenuation through modulation of indirect microbiome interactions.NPJ biofilms and microbiomes · 2024Article
- A Potential Intracanal Medicament, 2-Hydroxyisocaproic Acid (HICA): Cytotoxicity, Genotoxicity, and Its Effect on SCAP Differentiation.Dentistry journal · 2023Article
- Biochemical, functional and genomic characterization of a new probiotic Ligilactobacillus salivarius F14 from the gut of tribes of Odisha.World journal of microbiology & biotechnology · 2023Article
- Commensal Lactobacilli Metabolically Contribute to Cervical Epithelial Homeostasis in a Species-Specific Manner.mSphere · 2023Article
- Article
- Comprehensive analysis of metabolites produced by co-cultivation ofFrontiers in microbiology · 2023Article
- Antibacterial efficacy of sodium dichloroisocyanurate and 2-hydroxyisocaproic acid intracanal medicaments on Enterococcus faecalis: A comparative in-vitro study.Journal of oral biology and craniofacial researchArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The exploitation of natural antimicrobial compounds that can be used in food preservation has been fast tracked by the development of antimicrobial resistance to existing antimicrobials and the increasing consumer demand for natural food preservatives. 2-hydroxyisocaproic acid (HICA) is a natural compound produced through the leucine degradation pathway and is produced in humans and by certain microorganisms such as lactic acid bacteria and Clostridium species. The present study investigated the antibacterial efficacy of HICA against some important bacteria associated with food quality and safety and provided some insights into its possible antimicrobial mechanisms against bacteria. The results revealed that HICA was effective in inhibiting the growth of tested Gram-positive and Gram-negative bacteria including a multi-drug resistant P. aeruginosa strain in this study. The underlying mechanism was investigated by measuring the cell membrane integrity, membrane permeability, membrane depolarisation, and morphological and ultrastructural changes after HICA treatment in bacterial cells. The evidence supports that HICA exerts its activity via penetration of the bacterial cell membranes, thereby causing depolarisation, rupture of membranes, subsequent leakage of cellular contents and cell death. The current study suggests that HICA has potential to be used as an antibacterial agent against food spoilage and food-borne pathogenic bacteria, targeting the bacterial cell envelope.
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