Evidence map›Paper›PMID 35363444›Full record

ArticleJournal of veterinary science2022

Evaluation of porcine urine-derived cells as nuclei donor for somatic cell nuclear transfer.

Yu-Ting Zhang, Wang Yao, Meng-Jia Chai, Wen-Jing Liu, Yan Liu, Zhong-Hua Liu, Xiao-Gang Weng

Open access · goldAbstract read
In one paragraph

Article in Journal of veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yu-Ting ZhangKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, P.R. China.ORCID https://orcid.org/0000-0001-6202-1593
Wang YaoKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, P.R. China.ORCID https://orcid.org/0000-0002-4052-1981
Meng-Jia ChaiKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, P.R. China.ORCID https://orcid.org/0000-0002-4626-9713
Wen-Jing LiuKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, P.R. China.ORCID https://orcid.org/0000-0002-3919-9366
Yan LiuKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, P.R. China.ORCID https://orcid.org/0000-0002-8509-6325
Zhong-Hua LiuKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, P.R. China. liuzhonghua@neau.edu.cn.ORCID https://orcid.org/0000-0002-9199-6493
Xiao-Gang WengKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, P.R. China. wengxg@neau.edu.cn.ORCID https://orcid.org/0000-0002-7474-8447
Northeast Agricultural University · CN

Funding

Heilongjiang Provincial Postdoctoral Science Foundation LBH-Z17010Natural Science Foundation of Heilongjiang Province C2017035
6 · The paper itself

Abstract

backgroundSomatic cell nuclear transfer (SCNT) is used widely in cloning, stem cell research, and regenerative medicine. The type of donor cells is a key factor affecting the SCNT efficiency.

objectivesThis study examined whether urine-derived somatic cells could be used as donors for SCNT in pigs.

methodsThe viability of cells isolated from urine was assessed using trypan blue and propidium iodide staining. The H3K9me3/H3K27me3 level of the cells was analyzed by immunofluorescence. The

resultsMost sow urine-derived cells were viable and could be cultured and propagated easily. On the other hand, most of the somatic cells isolated from the boar urine exhibited poor cellular activity. The

conclusionsSow urine-derived cells could be used to produce SCNT embryos.

Indexed as

Cloning, OrganismNuclear Transfer TechniquesAnimalsBlastocystEmbryo TransferFemaleFibroblastsMaleSwineembryonic developmentPigsomatic cell nuclear transferurine

Identifiers

PMID35363444
PMCPMC8977534
OpenAlexW4223464482

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.