Evidence map›Paper›PMID 35362745›Full record

ArticleParasitology research2022

28S rRNA sequences for Linguatula spp.

Shokoofeh Shamsi, Xiaocheng Zhu, Ali Halajian, Diane P Barton

Open access · hybridAbstract read
In one paragraph

Article in Parasitology research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Exploring the anatomy ofInternational journal for parasitology. Parasites and wildlife · 2024
    Article
  4. Open veterinary journal · 2023
    Review
  5. Pathogens (Basel, Switzerland) · 2022
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Shokoofeh ShamsiSchool of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, 2678, Australia.ORCID http://orcid.org/0000-0002-8606-6400
Xiaocheng ZhuSchool of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, 2678, Australia.ORCID http://orcid.org/0000-0003-4468-1090
Ali HalajianDSI-NRF SARChI Chair (Ecosystem Health), Department of Biodiversity, University of Limpopo, Sovenga, South Africa.ORCID http://orcid.org/0000-0003-2279-940X
Diane P BartonSchool of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, 2678, Australia. dibarton@csu.edu.au.ORCID http://orcid.org/0000-0002-7462-9501
Charles Sturt University · AUUniversity of Limpopo · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identification of specimens belonging to the genus Linguatula (Pentastomida) is relatively easy due to their unique morphology. However, differentiation between species of Linguatula can be challenging for several reasons, including considerable differences between different developmental stages of the parasite within and between species. Currently, 18S rRNA and Cox1 sequences are the only available comparable sequences in GenBank, but recent research has discussed the utility of 28S rRNA for pentastomid phylogenetics. This study presents 28S rRNA gene sequences for two members of the genus Linguatula. Sequences of 28S rRNA were successfully obtained from well-identified samples of L. serrata (collected in Australia) and L. nuttalli (collected in South Africa), with voucher specimens. Phylogenetic analysis of the 28S rRNA region showed 6% difference between L. serrata and L. nuttalli, with low levels of intraspecific variation. In comparison, 18S rRNA and Cox1 sequences from the same specimens showed 0.23% and 13% interspecific differences, respectively. The results of this study show that 28S rRNA has greater genetic diversity to allow for improved differentiation between species of Linguatula than 18S rRNA but is on par with Cox1. Records that do not provide adequate morphological or molecular data to justify independent specific diagnoses must be regarded cautiously, and the need for continued research on species of Linguatula, using a combined morphological and molecular analysis, across a number of different hosts, development stages, geographical regions and molecular markers is highlighted.

Indexed as

PentastomidaAnimalsPhylogenyRNA, Ribosomal, 18SRNA, Ribosomal, 28SSouth AfricaRNA, Ribosomal, 18SRNA, Ribosomal, 28SLinguatula nuttalliLinguatula serrataMolecular sequencesPentastomidaPhylogeny

Identifiers

PMID35362745
PMCPMC9098581
OpenAlexW4220822631

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.