ArticlePlant methods2022
An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in peach reproductive tissues.
Article in Plant methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 12 citations in OpenAlex.
- Chromatin Immunoprecipitation in Crops.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Unlocking male sterility in horticultural crops through gene editing technology for precision breeding applications: presentation of a case study in tomato.Frontiers in plant science · 2025Article
- Dissecting postharvest chilling injuries in pome and stone fruit through integrated omics.Frontiers in plant science · 2023Review
- Advancing tree genomics to future proof next generation orchard production.Frontiers in plant science · 2023Review
- Evidence of chromatin and transcriptional dynamics for cold development in peach flower bud.The New phytologist · 2022Article
- ChIP-MS in Plant Systems: Mapping the H3K27ac Proteome During the Greening Process.Physiologia plantarumArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
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Abstract
backgroundPerennial fruit trees display a growth behaviour characterized by annual cycling between growth and dormancy, with complex physiological features. Rosaceae fruit trees represent excellent models for studying not only the fruit growth/patterning but also the progression of the reproductive cycle depending upon the impact of climate conditions. Additionally, current developments in high-throughput technologies have impacted Rosaceae tree research while investigating genome structure and function as well as (epi)genetic mechanisms involved in important developmental and environmental response processes during fruit tree growth. Among epigenetic mechanisms, chromatin remodelling mediated by histone modifications and other chromatin-related processes play a crucial role in gene modulation, controlling gene expression. Chromatin immunoprecipitation is an effective technique to investigate chromatin dynamics in plants. This technique is generally applied for studies on chromatin states and enrichment of post-transcriptional modifications (PTMs) in histone proteins.
resultsPeach is considered a model organism among climacteric fruits in the Rosaceae family for studies on bud formation, dormancy, and organ differentiation. In our work, we have primarily established specific protocols for chromatin extraction and immunoprecipitation in reproductive tissues of peach (Prunus persica). Subsequently, we focused our investigations on the role of two chromatin marks, namely the trimethylation of histone H3 at lysine in position 4 (H3K4me3) and trimethylation of histone H3 at lysine 27 (H3K27me3) in modulating specific gene expression. Bud dormancy and fruit growth were investigated in a nectarine genotype called Fantasia as our model system.
conclusionsWe present general strategies to optimize ChIP protocols for buds and mesocarp tissues of peach and analyze the correlation between gene expression and chromatin mark enrichment/depletion. The procedures proposed may be useful to evaluate any involvement of histone modifications in the regulation of gene expression during bud dormancy progression and core ripening in fruits.
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