Evidence map›Paper›PMID 35359999›Full record

ArticleFrontiers in immunology2022

Three-Dimensional Visualization With Tissue Clearing Uncovers Dynamic Alterations of Renal Resident Mononuclear Phagocytes After Acute Kidney Injury.

Kipyo Kim, Yun-Gyeong Kim, Su Woong Jung, Yang Gyun Kim, Sang-Ho Lee, Seung-Hae Kwon, Ju-Young Moon

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Kipyo KimDivision of Nephrology and Hypertension, Department of Internal Medicine, Inha University School of Medicine, Incheon, South Korea.
Yun-Gyeong KimDivision of Nephrology, Department of Internal Medicine, Kyung Hee University, College of Medicine, Seoul, South Korea.
Su Woong JungDivision of Nephrology, Department of Internal Medicine, Kyung Hee University, College of Medicine, Seoul, South Korea.
Yang Gyun KimDivision of Nephrology, Department of Internal Medicine, Kyung Hee University, College of Medicine, Seoul, South Korea.
Sang-Ho LeeDivision of Nephrology, Department of Internal Medicine, Kyung Hee University, College of Medicine, Seoul, South Korea.
Seung-Hae KwonKorea Basic Science Institute, Seoul Center, Seoul, South Korea.
Ju-Young MoonDivision of Nephrology, Department of Internal Medicine, Kyung Hee University, College of Medicine, Seoul, South Korea.
Kyung Hee University · KRInha University · KRKorea Basic Science Institute · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traditional histologic methods are limited in detecting dynamic changes in immune cells during acute kidney injury (AKI). Recently, optical tissue clearing combined with multiphoton microscopy (MPM) or light sheet fluorescence microscopy (LSFM) has become an emerging method for deep tissue evaluation and three-dimensional visualization. These new approaches have helped expand our understanding of tissue injury and repair processes, including tracing the changes in immune cells. We designed this study to investigate the morphological and functional alterations of renal mononuclear phagocytes (MNPs) in lipopolysaccharide (LPS)-induced AKI using renal clearing in CD11c-YFP mice. We also evaluated the effect of the NLRP3 inhibitor MCC950 to determine whether NLRP3 inhibition attenuates the activation of CD11c+ cells in an LPS-induced AKI model. Transverse sectioned whole mouse kidney imaging by LSFM showed that CD11c+ cells were mainly distributed in the cortex, especially the tubulointerstitial area. The number of CD11c+ cells was significantly more densely interspersed, particularly in periglomerular and perivascular lesions, in the saline-treated LPS-exposed kidney than in the control kidney. Deep imaging of the kidney cortex by MPM demonstrated an increased number of CD11c+ cells in the saline-treated LPS group compared with the control group. This quantitative alteration of CD11c+ cells in AKI was accompanied by morphological changes at high resolution, showing an increased number and level of dendrites. These morphological and behavioral changes in the saline-treated LPS group were accompanied by increased MHC class II and CD86 on CD11c-YFP+ cells. MCC950 attenuated the activation of CD11c+ cells after AKI and improved renal function. In conclusion, wide and deep three-dimensional visualization using MPM or LSFM combined with kidney clearing uncovers dynamic changes of renal MNPs, which are directly linked to renal function in AKI.

Indexed as

Acute Kidney InjuryLipopolysaccharidesAnimalsImaging, Three-DimensionalKidneyMiceNLR Family, Pyrin Domain-Containing 3 ProteinPhagocytesLipopolysaccharidesNLR Family, Pyrin Domain-Containing 3 Proteinacute kidney injuryinnate immunitylight sheet fluorescence microscopymononuclear phagocytetissue clearing methods

Identifiers

PMID35359999
PMCPMC8960144
OpenAlexW4220662997

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.