Evidence map›Paper›PMID 35359324›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Analysis of DNA Double-Stranded Breaks Using the Comet Assay in Planarians.

Paul G Barghouth, Salvador Rojas, Lacey R O'Dell, Andrew M Betancourt, Néstor J Oviedo

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Paul G BarghouthDepartment of Molecular & Cell Biology, University of California, Merced, CA, USA.
Salvador RojasDepartment of Molecular & Cell Biology, University of California, Merced, CA, USA.
Lacey R O'DellDepartment of Molecular & Cell Biology, University of California, Merced, CA, USA.
Andrew M BetancourtDepartment of Molecular & Cell Biology, University of California, Merced, CA, USA.
Néstor J OviedoDepartment of Molecular & Cell Biology, University of California, Merced, CA, USA. noviedo2@ucmerced.edu.
University of California, Merced · US

Funding

The Effects of Direct Current Stimulation in Adult TissuesR01GM132753 · NIGMS · UNIVERSITY OF CALIFORNIA, MERCED · PI OVIEDO, NESTOR J · 2019 to 2022
$1.2M
NIGMS NIH HHS R01 GM132753
6 · The paper itself

Abstract

Comet assay provides the opportunity to detect and characterize DNA strand breaks. Cellular lysing followed by embedding in agarose slide is used to visualize under an electrical current migration patterns corresponding to DNA fragments of different sizes. Here we describe the process of detecting and characterizing DNA damage by Comet assay on planarians, which is a model organism commonly used to understand the process of whole-body regeneration, stem cell regulation, and adult tissue maintenance.

Indexed as

PlanariansAnimalsComet AssayDNADNA Breaks, Double-StrandedDNA DamageDNAAlkaline Comet AssayDNA damageDouble-stranded breaksPlanarian

Identifiers

PMID35359324
PMCPMC9761910
OpenAlexW4225929905

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.