Evidence map›Paper›PMID 35359313›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Studying Echinodermata Arm Explant Regeneration Using Echinaster sepositus.

Cinzia Ferrario, Yousra Ben Khadra, Michela Sugni, M Daniela Candia Carnevali, Pedro Martinez, Francesco Bonasoro

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Cinzia FerrarioDepartment of Environmental Science and Policy, University of Milan, Milan, Italy.ORCID https://orcid.org/0000-0002-1804-4760
Yousra Ben KhadraLaboratory of Genetics, Biodiversity, and Valorization of Bioresources, Higher Institute of Biotechnology, University of Monastir, Monastir, Tunisia.ORCID https://orcid.org/0000-0002-0669-4811
Michela SugniDepartment of Environmental Science and Policy, University of Milan, Milan, Italy. michela.sugni@unimi.it.ORCID https://orcid.org/0000-0002-4574-5802
M Daniela Candia CarnevaliDepartment of Environmental Science and Policy, University of Milan, Milan, Italy.ORCID https://orcid.org/0000-0002-0215-8843
Pedro MartinezDepartment of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain.ORCID https://orcid.org/0000-0003-3956-7541
Francesco BonasoroDepartment of Environmental Science and Policy, University of Milan, Milan, Italy.ORCID https://orcid.org/0000-0002-1559-0707

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Echinoderms are marine invertebrate deuterostomes known for their amazing regenerative abilities throughout all life stages. Though some species can undergo whole-body regeneration (WBR), others exhibit more restricted regenerative capabilities. Asteroidea (starfish) comprise one of the few echinoderm taxa capable of undergoing WBR. Indeed, some starfish species can restore all tissues and organs not only during larval stages, but also from arm fragments as adults. Arm explants have been used to study cells, tissues and genes involved in starfish regeneration. Here, we describe methods for obtaining and studying regeneration of arm explants in starfish, in particular animal collection and husbandry, preparation of arm explants, regeneration tests, microscopic anatomy techniques (including transmission electron microscopy, TEM) used to analyze the regenerating explant tissues and cells plus a downstream RNA extraction protocol needed for subsequent molecular investigations.

Indexed as

EchinodermataStarfishAnimalsLarvaArm explantsEchinodermsRegenerationRNA extractionSemithin and ultrathin sectioningStarfishTEM grid stainingTransmission electron microscopy

Identifiers

PMID35359313
PMCPMC9761906

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.