ArticlePlant physiology2022
Alkaline α-galactosidase 2 (CsAGA2) plays a pivotal role in mediating source-sink communication in cucumber.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 30 citations in OpenAlex.
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- Silencing of the Alkaline α-Galactosidase GeneInternational journal of molecular sciences · 2025Article
- Raffinose family oligosaccharide hydrolysis by alkaline α-galactosidase CsAGA2 controls seed development in cucumber.The Plant cell · 2025Article
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- Virus-InducedPlants (Basel, Switzerland) · 2024Article
- Significance of Raffinose Family Oligosaccharides (RFOs) metabolism in plants.Advanced biotechnology · 2024Review
- Genome-wide identification and expression analysis of the WNK kinase gene family in soybean.Molecular breeding : new strategies in plant improvement · 2024Article
- The Sink-Source Relationship in Cucumber (Plants (Basel, Switzerland) · 2023Article
- Soybean reduced internode 1 determines internode length and improves grain yield at dense planting.Nature communications · 2023Article
- QTL Mapping and Genome-Wide Association Study Reveal Genetic Loci and Candidate Genes Related to Soluble Solids Content in Melon.Current issues in molecular biology · 2023Article
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- Far-Red Light Mediated Carbohydrate Concentration Changes in Leaves of Sweet Basil, a Stachyose Translocating Plant.International journal of molecular sciences · 2023Article
- Optimizing raffinose family oligosaccharides content in plants: A tightrope walk.Frontiers in plant science · 2023Review
- Morphological and Genetic Diversity of Cucumber (Plants (Basel, Switzerland) · 2022Review
- A Comparative Characterization and Expression Profiling Analysis ofInternational journal of molecular sciences · 2022Article
- Combined analysis of the transcriptome and metabolome provides insights into the fleshy stem expansion mechanism in stem lettuce.Frontiers in plant science · 2022Article
- A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition.Frontiers in plant science · 2022Article
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sugars are necessary for plant growth and fruit development. Cucumber (Cucumis sativus L.) transports sugars, mainly raffinose family oligosaccharides (RFOs), in the vascular bundle. As the dominant sugars in cucumber fruit, glucose and fructose are derived from sucrose, which is the product of RFO hydrolysis by α-galactosidase (α-Gal). Here, we characterized the cucumber alkaline α-galactosidase 2 (CsAGA2) gene and found that CsAGA2 has undergone human selection during cucumber domestication. Further experiments showed that the expression of CsAGA2 increases gradually during fruit development, especially in fruit vasculature. In CsAGA2-RNA interference (RNAi) lines, fruit growth was delayed because of lower hexose production in the peduncle and fruit main vascular bundle (MVB). In contrast, CsAGA2-overexpressing (OE) plants displayed bigger fruits. Functional enrichment analysis of transcriptional data indicated that genes related to sugar metabolism, cell wall metabolism, and hormone signaling were significantly downregulated in the peduncle and fruit MVBs of CsAGA2-RNAi plants. Moreover, downregulation of CsAGA2 also caused negative feedback regulation on source leaves, which was shown by reduced photosynthetic efficiency, fewer plasmodesmata at the surface between mesophyll cell and intermediary cell (IC) or between IC and sieve element, and downregulated gene expression and enzyme activities related to phloem loading, as well as decreased sugar production and exportation from leaves and petioles. The opposite trend was observed in CsAGA2-OE lines. Overall, we conclude that CsAGA2 is essential for cucumber fruit set and development through mediation of sugar communication between sink strength and source activity.
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