ArticleBiology of reproduction2022
Spatial transcriptomic profiles of mouse uterine microenvironments at pregnancy day 7.5†.
Article in Biology of reproduction, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Mesenchymal-to-epithelial transition of perivascular cells contributes to endometrial re-epithelialization.Nature communications · 2025Article
- Cell type and cell signalling innovations underlying mammalian pregnancy.Nature ecology & evolution · 2025Article
- An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling.Nature communications · 2025Article
- Innate Lymphoid Cells in Reproductive Health and Disease.European journal of immunology · 2025Review
- The maternal-fetal interface at single-cell resolution: uncovering the cellular anatomy of the placenta and decidua.American journal of obstetrics and gynecology · 2025Review
- Progesterone receptor in uterine glands is required for pregnancy establishment in mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Revolutionizing Implantation Studies: Uterine-Specific Models and Advanced Technologies.Biomolecules · 2025Review
- Signaling via retinoic acid receptors mediates decidual angiogenesis in mice and human stromal cell decidualization.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Spatiotemporally distinct roles of cyclooxygenase-1 and cyclooxygenase-2 at fetomaternal interface in mice.JCI insight · 2024Article
- PR-SET7 epigenetically restrains uterine interferon response and cell death governing proper postnatal stromal development.Nature communications · 2024Article
- The single-cell atlas of the epididymis in mice reveals the changes in epididymis function before and after sexual maturity.Frontiers in cell and developmental biology · 2024Article
- Exploring distinct properties of endometrial stem cells through advanced single-cell analysis platforms.Stem cell research & therapy · 2023Review
- ATOH8 Expression Is Regulated by BMP2 and Plays a Key Role in Human Endometrial Stromal Cell Decidualization.Endocrinology · 2023Article
- Dissecting mammalian reproduction with spatial transcriptomics.Human reproduction update · 2023Review
- Vascular changes in the cycling and early pregnant uterus.JCI insight · 2023Review
- The NR2F2-HAND2 signaling axis regulates progesterone actions in the uterus at early pregnancy.Frontiers in endocrinology · 2023Article
- Single-Cell Immunobiology of the Maternal-Fetal Interface.Journal of immunology (Baltimore, Md. : 1950) · 2022Review
- Myometrial progesterone receptor determines a transcription program for uterine remodeling and contractions during pregnancy.PNAS nexus · 2022Article
- The role of extravillous trophoblasts and uterine NK cells in vascular remodeling during pregnancy.Frontiers in immunology · 2022Review
- Killer Timing: The Temporal Uterine Natural Killer Cell Differentiation Pathway and Implications for Female Reproductive Health.Frontiers in endocrinology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Uterine dysfunctions lead to fertility disorders and pregnancy complications. Normal uterine functions at pregnancy depend on crosstalk among multiple cell types in uterine microenvironments. Here, we performed the spatial transcriptomics and single-cell RNA-seq assays to determine local gene expression profiles at the embryo implantation site of the mouse uterus on pregnancy day 7.5 (D7.5). The spatial transcriptomic annotation identified 11 domains of distinct gene signatures, including a mesometrial myometrium, an anti-mesometrial myometrium, a mesometrial decidua enriched with natural killer cells, a vascular sinus zone for maternal vessel remodeling, a fetal-maternal interface, a primary decidual zone, a transition decidual zone, a secondary decidual zone, undifferentiated stroma, uterine glands, and the embryo. The scRNA-Seq identified 12 types of cells in the D7.5 uterus including three types of stromal fibroblasts with differentiated and undifferentiated markers, one cluster of epithelium including luminal and glandular epithelium, mesothelium, endothelia, pericytes, myelomonocytic cell, natural killer cells, and lymphocyte B. These single-cell RNA signatures were then utilized to deconvolute the cell-type compositions of each individual uterine microenvironment. Functional annotation assays on spatial transcriptomic data revealed uterine microenvironments with distinguished metabolic preferences, immune responses, and various cellular behaviors that are regulated by region-specific endocrine and paracrine signals. Global interactome among regions is also projected based on the spatial transcriptomic data. This study provides high-resolution transcriptome profiles with locality information at the embryo implantation site to facilitate further investigations on molecular mechanisms for normal pregnancy progression.
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