Evidence map›Paper›PMID 35356814›Full record

ArticleBioactive materials2022

Peptide ligand-SiO

Kaige Yang, Mengqi Jia, Soumia Cheddah, Zhouyi Zhang, Weiwei Wang, Xinyan Li, Yan Wang, Chao Yan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioactive materials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 38 citations in OpenAlex.

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  18. Organism-Generated Biological Vesicles In Situ: An Emerging Drug Delivery Strategy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Review
  19. Recent developments in isolating methods for exosomes.Frontiers in bioengineering and biotechnology · 2022
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Kaige YangSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Mengqi JiaDepartment of Clinical Pharmacy, Shanghai General of Hospital, Shanghai Jiao Tong University, Shanghai, 200240, China.
Soumia CheddahSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Zhouyi ZhangSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Weiwei WangSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Xinyan LiSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Yan WangSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Chao YanSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Shanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes are membrane bound extracellular vesicles that play an important role in many biological processes. While they have great application value, exosome isolation is still considered a major scientific challenge. In the present study, a novel separation strategy for exosomes is proposed based on the specific interaction between immobilized peptide ligands and phosphatidylserine moieties which are highly abundant on the surface of exosomes. With the new affinity method, intact model exosomes can be recovered with a high yield in a short processing time. The purity of exosome samples enriched from serum by the affinity method is far higher than that isolated by ultrafiltration, and similar to that obtained by density gradient centrifugation and ultracentrifugation. Moreover, the variety of contaminants co-isolated by the affinity method is relatively low due to its specific separation principle. Proteomics analysis of exosomes isolated by the affinity method from the serum of healthy, hepatocellular carcinoma patients, and intrahepatic cholangiocarcinoma patients was performed to prove the applicability of this method. In conclusion, our novel strategy shows characteristics of easy preparation, high specificity, and cost-effectiveness, and provides a promising approach for exosome isolation which should have wide applications.

Indexed as

ExosomeHepatic cancerIsolationPeptide ligandPhosphatidylserineProteomics

Identifiers

PMID35356814
PMCPMC8935132
OpenAlexW4200346650

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.