Evidence map›Paper›PMID 35354913›Full record

ArticleExperimental & molecular medicine2022

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes.

Long-Fei Wu, Qin Zhang, Xing-Bo Mo, Jun Lin, Yang-Lin Wu, Xin Lu, Pei He, Jian Wu, Yu-Fan Guo, Ming-Jun Wang and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental & molecular medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 2 pooled it
5.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 2 syntheses or guidelines pooled it, 64 citations in OpenAlex.

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  20. Clinical significance of long non-coding RNA NORAD in rheumatoid arthritis.Advances in rheumatology (London, England) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 2 countries.

Long-Fei WuCenter for Genetic Epidemiology and Genomics, School of Public Health, Medical College of Soochow University, 215123, Suzhou, Jiangsu, China.
Qin ZhangDepartment of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Xing-Bo MoCenter for Genetic Epidemiology and Genomics, School of Public Health, Medical College of Soochow University, 215123, Suzhou, Jiangsu, China.
Jun LinDepartment of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Yang-Lin WuDepartment of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Xin LuCenter for Genetic Epidemiology and Genomics, School of Public Health, Medical College of Soochow University, 215123, Suzhou, Jiangsu, China.
Pei HeCenter for Genetic Epidemiology and Genomics, School of Public Health, Medical College of Soochow University, 215123, Suzhou, Jiangsu, China.
Jian WuDepartment of Rheumatology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Yu-Fan GuoDepartment of Rheumatology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Ming-Jun WangDepartment of Rheumatology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Wen-Yan RenCam-Su Genomic Resource Center, Medical College of Soochow University, 215123, Suzhou, Jiangsu, China.
Hong-Wen DengCenter of Bioinformatics and Genomics, Department of Global Biostatistics and Data Science, Tulane University, New Orleans, LA, USA.
Shu-Feng LeiCenter for Genetic Epidemiology and Genomics, School of Public Health, Medical College of Soochow University, 215123, Suzhou, Jiangsu, China. leisf@suda.edu.cn.
Fei-Yan DengCenter for Genetic Epidemiology and Genomics, School of Public Health, Medical College of Soochow University, 215123, Suzhou, Jiangsu, China. fdeng@suda.edu.cn.
Soochow University · CNFirst Affiliated Hospital of Soochow University · CNTulane University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

Indexed as

Arthritis, ExperimentalArthritis, RheumatoidExosomesMicroRNAsAnimalsCell ProliferationFibroblastsHumansMiceSynovial MembraneMicroRNAsMIRN204 microRNA, human

Identifiers

PMID35354913
PMCPMC8980013
OpenAlexW4220905659

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.