Evidence map›Paper›PMID 35353294›Full record

ArticleInternational ophthalmology2022

C-terminal binding protein 2 promotes high-glucose-triggered cell proliferation, angiogenesis and cellular adhesion of human retinal endothelial cell line.

Han Li, Feng Zhou, Xingjian Cao, Ping Zhao, Xiaobo Huang, Wei Xie, Guowei Zhang, Xiang Chen

Abstract read
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In one paragraph

Article in International ophthalmology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Han Li *Department of Clinical Laboratory, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Feng Zhou *Department of Clinical Laboratory, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Xingjian CaoDepartment of Clinical Laboratory, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Ping ZhaoDepartment of Clinical Laboratory, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Xiaobo HuangDepartment of Ophthalmology, The Second Affiliated Hospital of Nantong University, Nantong, China.
Wei XieDepartment of Clinical Laboratory, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Guowei ZhangEye Institute, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China. zgweyelab@163.com.ORCID http://orcid.org/0000-0001-6059-1947
Xiang ChenDepartment of Clinical Laboratory, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China. ntchenx0520@163.com.
Nantong University · CN

Funding

National Natural Science Foundation of China 81500706Postdoctoral Research Foundation of China 2020M671562Postdoctoral Science Foundation of Jiangsu Province 2020Z318Science and Technology Project of Nantong Municipality HS2020005Science and Technology Project of Nantong Municipality YYZ17010
6 · The paper itself

Abstract

purposeThe proliferation and angiogenesis of human retinal endothelial cells (HRECs) are critical for the pathophysiology of diabetic retinopathy (DR). C-terminal binding protein 2 (CtBP2) has multiple biologic functions, but its effect on HRECs under high-glucose (HG) conditions is unclear.

methodsThe cell viability, angiogenesis, cellular adhesion and CtBP2 expression levels of HRECs were measured following treatment with different concentrations of glucose. Small interfering CtBP2-targeting RNA, wide-type and function mutant plasmid of CtBP2 were constructed and then were transfected into HRECs to evaluate the effects of CtBP2 on cell functions of HRECs.

resultsThe expression of CtBP2 in HRECs was increased after HG treatment. HG treatment significantly increased cell proliferation, angiogenesis, and decreased relative gene expressions in gap junctions, tight junctions and adherens junctions. After CtBP2 was inhibited via siRNA, the changes induced by HG were partially restored. Conversely, only wild-type CtBP2 could increase cell proliferation and angiogenesis under HG condition. Mechanistically, we also found that CtBP2 exerted its functions to effect HG-induced changes via Akt signaling pathway.

conclusionThis study implicates that CtBP2 promotes HG-induced cell proliferation, angiogenesis and cellular adhesion, and CtBP2 might be a potential target in the prevention of DR.

Indexed as

Biological ProductsDiabetic RetinopathyAlcohol OxidoreductasesCell ProliferationDNA-Binding ProteinsEndothelial CellsGlucoseHumansNeovascularization, PathologicProto-Oncogene Proteins c-aktRetinaRNA, Small InterferingAlcohol OxidoreductasesBiological ProductsC-terminal binding proteinDNA-Binding ProteinsGlucoseProto-Oncogene Proteins c-aktRNA, Small InterferingAngiogenesisCellular adhesionCtBP2Diabetic retinopathyHigh glucoseHuman retinal endothelial cells

Identifiers

PMID35353294
OpenAlexW4220933028

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.