ArticleEnvironmental microbiology2022
Mushroom-shaped structures formed in Acinetobacter baumannii biofilms grown in a roller bioreactor are associated with quorum sensing-dependent Csu-pilus assembly.
Article in Environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 21 citations in OpenAlex.
- Industrial WWTP Sludge as a Reservoir of Putative Plastic-Degrading Enzymes: Insights Into Microbial Community Structure.Microbial ecology · 2026Article
- Therapeutic significance of surface-exposed virulence factors in Acinetobacter baumannii pathogenicity.Molecular biology reports · 2026Review
- Network-level divergence in cyclic di-GMP signalling drives ecological versatility in Acinetobacter baumannii.NPJ biofilms and microbiomes · 2026Article
- Antiparallel stacking of Csu pili drives Acinetobacter baumannii 3D biofilm assembly.Nature communications · 2026Article
- AHL-based QS signalling promotes uropathogenic Escherichia coli settlement through the de-repression of biofilm formation by SdiA.PloS one · 2025Article
- Regulatory effects on virulence and phage susceptibility revealed byFrontiers in cellular and infection microbiology · 2025Article
- Cyclic AMP is a global virulence regulator governing inter and intrabacterial signalling in Acinetobacter baumannii.PLoS pathogens · 2024Article
- Triblock copolymer micelles enhance solubility, permeability and activity of a quorum sensing inhibitor againstRSC applied polymers · 2024Article
- Multitasking functions of bacterial extracellular DNA in biofilms.Journal of bacteriology · 2024Review
- Ciprofloxacin Poly(β-amino ester) Conjugates Enhance Antibiofilm Activity and Slow the Development of Resistance.ACS applied materials & interfaces · 2024Article
- Colony phase variation switch modulates antimicrobial tolerance and biofilm formation inMicrobiology spectrum · 2024Article
- Design, Synthesis, and Evaluation of New 1Journal of medicinal chemistry · 2024Article
- A microtiter peg lid with ziggurat geometry for medium-throughput antibiotic testing andBiofilm · 2023Article
- Csu pili dependent biofilm formation and virulence of Acinetobacter baumannii.NPJ biofilms and microbiomes · 2023Article
- Toward Comprehensive Analysis of the 3D Chemistry ofAnalytical chemistry · 2023Article
- Isolation and characterization of a VHH targeting the Acinetobacter baumannii cell surface protein CsuA/B.Applied microbiology and biotechnology · 2023Article
- Design of Quorum Sensing Inhibitor-Polymer Conjugates to PenetrateACS macro letters · 2023Article
- Artificial sweeteners inhibit multidrug-resistant pathogen growth and potentiate antibiotic activity.EMBO molecular medicine · 2023Article
- Carbapenem-ResistantMicroorganisms · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
There is currently a need to develop simple biofilm models that facilitate investigation of the architecture/biology of mature bacterial biofilms in a consistent/standardized manner given their environmental and clinical importance and the need for new anti-biofilm interventions. This study introduces a novel biofilm culture system termed the rolling biofilm bioreactor (RBB). This easily operated system allows adherent microbial cells to be repeatedly exposed to air/solid/liquid interfaces optimizing biofilm growth. The RBB was exploited to investigate biofilm formation in Acinetobacter baumannii. High levels of A. baumannii biofilm biomass reproducibly accumulate in the RBB and, importantly, undergo a maturation step to form large mushroom-shaped structures that had not been observed in other models. Based on image analysis of biofilm development and genetic manipulation, we show how N-acylhomoserine lactone-dependent quorum sensing (QS) impacts on biofilm differentiation, composition and antibiotic tolerance. Our results indicate that extracellular DNA (eDNA) is a key matrix component in mature Acinetobacter biofilms as the mushroom-like structures consist of dense cellular masses encased in an eDNA mesh. Moreover, this study reveals the contribution of QS to A. baumannii biofilm differentiation through Csu pilus assembly regulation. Understanding the mechanisms of structural development of mature biofilms helps to identify new biofilm eradication and removal strategies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.