ArticleJournal of structural biology2022
Exploring high-resolution cryo-ET and subtomogram averaging capabilities of contemporary DEDs.
Article in Journal of structural biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- A cryo-CLEM-guided workflow for quantitative assessment and optimization of cryo-ET specimen quality.Molecules and cells · 2026Article
- Automated removal of corrupted tilts in cryo-electron tomography.Journal of structural biology: X · 2025Article
- Template Learning: Deep learning with domain randomization for particle picking in cryo-electron tomography.Nature communications · 2025Article
- A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries.Current opinion in structural biology · 2025Review
- Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice.Nature structural & molecular biology · 2025Article
- Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data.Journal of visualized experiments : JoVE · 2025Article
- CryoVesNet: A dedicated framework for synaptic vesicle segmentation in cryo-electron tomograms.The Journal of cell biology · 2025Article
- Overcoming the preferred-orientation problem in cryo-EM with self-supervised deep learning.Nature methods · 2025Article
- Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024Review
- Overcoming the preferred orientation problem in cryoEM with self-supervised deep-learning.bioRxiv : the preprint server for biology · 2024Article
- Unlocking cryo-EM's multishot potential with square or rectangular beams.Nature methods · 2024Article
- Conventional Electron Microscopy, Cryogenic Electron Microscopy, and Cryogenic Electron Tomography of Viruses.Sub-cellular biochemistry · 2024Review
- The big chill: Growth ofQRB discovery · 2024Review
- Deciphering the molecular mechanisms of actin cytoskeleton regulation in cell migration using cryo-EM.Biochemical Society transactions · 2023Review
- Ot2Rec: A semi-automatic, extensible, multi-software tomographic reconstruction workflow.Biological imaging · 2023Article
- Electron counting takes microED to the next level.Nature methods · 2022Article
- Subtomogram averaging for biophysical analysis and supramolecular context.Journal of structural biology: X · 2022Article
- CContact (Thousand Oaks (Ventura County, Calif.))Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
Abstract
The potential of energy filtering and direct electron detection for cryo-electron microscopy (cryo-EM) has been well documented. Here, we assess the performance of recently introduced hardware for cryo-electron tomography (cryo-ET) and subtomogram averaging (STA), an increasingly popular structural determination method for complex 3D specimens. We acquired cryo-ET datasets of EIAV virus-like particles (VLPs) on two contemporary cryo-EM systems equipped with different energy filters and direct electron detectors (DED), specifically a Krios G4, equipped with a cold field emission gun (CFEG), Thermo Fisher Scientific Selectris X energy filter, and a Falcon 4 DED; and a Krios G3i, with a Schottky field emission gun (XFEG), a Gatan Bioquantum energy filter, and a K3 DED. We performed constrained cross-correlation-based STA on equally sized datasets acquired on the respective systems. The resulting EIAV CA hexamer reconstructions show that both systems perform comparably in the 4-6 Å resolution range based on Fourier-Shell correlation (FSC). In addition, by employing a recently introduced multiparticle refinement approach, we obtained a reconstruction of the EIAV CA hexamer at 2.9 Å. Our results demonstrate the potential of the new generation of energy filters and DEDs for STA, and the effects of using different processing pipelines on their STA outcomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.