ArticleBMC biology2022
The repertoire of testicular extracellular vesicle cargoes and their involvement in inter-compartmental communication associated with spermatogenesis.
Article in BMC biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Altered sncRNA Signatures in Semen Extracellular Vesicles Between Patients with Benign and Malignant Prostate Disease as Potential Non-Invasive Biomarkers in the PSA Grey Zone.International journal of molecular sciences · 2026Article
- Seminal-Plasma Molecular Biomarkers as a Liquid Biopsy of Testicular Function: Toward AI-Ready Sperm-Retrieval Prediction in Non-Obstructive Azoospermia.International journal of molecular sciences · 2026Review
- Molecular Profiling of Extracellular Vesicles Isolated from Boar Reproductive Fluids.Journal of extracellular vesicles · 2026Article
- Seminal Plasma Exosomes in Male Infertility: Roles, Mechanisms, and Applications-A Double-Edged Sword.The world journal of men's health · 2026Review
- Sertoli Cell-Derived Extracellular Vesicles Orchestrate Cadmium-Induced Testicular Inflammation and Fibrosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Epididymal extracellular vesicles harbor and convey mRNA to sperm for transfer to zygotes.Nucleic acids research · 2026Article
- The Potential Roles of Extracellular Vesicles in Wild Animals' Reproduction.Advances in experimental medicine and biology · 2026Review
- Impaired antigen clearance of macrophages associates with heart failure and non-obstructive azoospermia: insights from transcriptomics, in silico knockout, andFrontiers in immunology · 2026Article
- Extracellular-vesicle-mediated transfer of let-7b/7c promotes the proliferation of transition-state spermatogonia in neonatal mouse testis.Stem cell reports · 2025Article
- Male reproductive tract extracellular vesicles display region-specific heterogeneity in mice.Reproduction (Cambridge, England) · 2025Article
- Engineered extracellular vesicles: a breakthrough approach to overcoming sperm cryopreservation challenges.Reproductive biology and endocrinology : RB&E · 2025Review
- Engineered nanoparticles potentials in male reproduction.Andrology · 2025Review
- Sperm-borne small non-coding RNAs: potential functions and mechanisms as epigenetic carriers.Cell & bioscience · 2025Review
- The Biology and Regulation of Spermatogonial Stem Cells in the Niche.Advances in experimental medicine and biology · 2025Review
- Extracellular vesicles: key mediators inFrontiers in veterinary science · 2025Review
- The extracellular vesicle tetraspanin CD63 journey from the testis through the epididymis to mature bull sperm.Scientific reports · 2024Article
- Semen sEV tRF-Based Models Increase Non-Invasive Prediction Accuracy of Clinically Significant Prostate Cancer among Patients with Moderately Altered PSA Levels.International journal of molecular sciences · 2024Article
- Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives.Molecular and cellular biochemistry · 2024Review
- Cadmium-induced impairment of spermatozoa development by reducing exosomal-MVBs secretion: a novel pathway.Aging · 2023Article
- The Sperm Small RNA Transcriptome: Implications beyond Reproductive Disorder.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpermatogenesis is regulated by a complex network of intercellular communication processes. Extracellular vesicles (EVs) are one of the important mediators in intercellular communication. Previous reports have demonstrated the involvement of EVs from the epididymis and prostate in sperm maturation and function. However, the presence of EVs in the testis and their potential involvement in spermatogenesis has not been explored. Here, we have established a testis dissociation protocol that allows the isolation and characterization of testicular EVs.
resultsWe show that testicular EVs are specifically and efficiently taken up by somatic cells and germ cells, including the spermatozoa in the interstitial space and the seminiferous tubule compartments. We profiled the proteome of testicular EVs and probed the cell types that release them, revealing the potential contributions from the Leydig cells and testicular macrophages. Moreover, we sequenced the small RNA cargoes of testicular EVs and identified sets of small non-coding RNAs that were overlooked in the testis transcriptome. Selected miRNA candidates in testicular EVs were found in sperm RNA payload and demonstrated specific resistance towards ribonuclease A independent of the vesicle membrane. Small molecule inhibition of EV secretion perturbed spermatogenesis via inter-compartmental communication.
conclusionsTogether, our study provides a valuable resource on the repertoire of cargoes carried by testicular EVs and uncovers a physiological function of testicular EVs in inter-compartmental communication associated to spermatogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.