Evidence map›Paper›PMID 35350642›Full record

ArticleIranian journal of biotechnology2021

Recombinant Expression, Purification and Characterization of Pyridoxal 5'-phosphate Synthase from

Adulwit Sinthusiri, Chamaiporn Champasri, Yanee Trongpanich

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Iranian journal of biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Adulwit SinthusiriDepartment of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Chamaiporn ChampasriDepartment of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Yanee TrongpanichDepartment of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Khon Kaen University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Pyridoxal 5' -phosphate synthase (PLPS) is present in deoxyxylose 5'-phosphate-independent of the Objectives: This study aimed to clone, express, and purify PLPS of Material and Methods: The Results: The molecular weights of rGhpdxS and rGhpdxT were estimated to be 35 and 23 kDa by SDS-PAGE, respectively. The native form of rGhpdxS showed hexamer and dodecamer, whereas those of rGhpdxT were a monomer upon detection with non-denaturing gel electrophoresis and gel filtration. A molar ratio of 1:1 of rGhpdxS:rGhpdxT showed the highest PLP synthesis activity (4.16 U.mg Conclusions: These results support the potential of rGhPLPS as a candidate for industrial application.

Indexed as

GeobacillusPyridoxal 5ʹ-phosphate synthaseRecombinant ProteinsThermophilic bacterium

Identifiers

PMID35350642
PMCPMC8926315
OpenAlexW3210797800

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.