Evidence map›Paper›PMID 35350330›Full record

ArticleACS omega2022

Suppressing Nonspecific Binding in Biolayer Interferometry Experiments for Weak Ligand-Analyte Interactions.

Alyssa Dubrow, Bryan Zuniga, Elias Topo, Jae-Hyun Cho

Open access · goldAbstract read
In one paragraph

Article in ACS omega, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Characterization of antiviral compounds using Bio-Layer Interferometry.bioRxiv : the preprint server for biology · 2025
    Article
  5. Article
  6. Article
  7. Article
  8. Evolutionary rewiring of the dynamic network underpinning allosteric epistasis in NS1 of the influenza A virus.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  9. A Tag-Free Platform for Synthesis and Screening of Cyclic Peptide Libraries.Angewandte Chemie (International ed. in English) · 2024
    Article
  10. Protein-Protein Binding Kinetics by Biolayer Interferometry.Advances in experimental medicine and biology · 2024
    Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Alyssa DubrowDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843, United States.
Bryan ZunigaDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843, United States.
Elias TopoDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843, United States.
Jae-Hyun ChoDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843, United States.ORCID https://orcid.org/0000-0002-6190-6151
Texas A&M University · US

Funding

Structure and functional dynamics of virus-host protein interactionsR01GM127723 · NIGMS · TEXAS A&M AGRILIFE RESEARCH · PI CHO, JAE HYUN · 2018 to 2022
$1.9M
NIGMS NIH HHS R01 GM127723
6 · The paper itself

Abstract

Quantitative analysis of protein-protein interactions (PPIs) using biolayer interferometry (BLI) requires effective suppression of nonspecific binding (NSB) between analytes and biosensors. In particular, the study of weak interactions (i.e.,

Identifiers

PMID35350330
PMCPMC8945191
OpenAlexW4220914888

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.