Evidence map›Paper›PMID 35349595›Full record

ArticlePloS one2022

Physio-biochemical responses and expressional profiling analysis of drought tolerant genes in new promising rice genotype.

Haytham A Freeg, Kotb A Attia, Stuart Casson, Sajid Fiaz, Ebrahim A Ramadan, Antar El- Banna, Nicholas Zoulias, Ali Aboshosha, Salman Alamery

RetractedAbstract readRetracted Publication
In one paragraph

Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Haytham A FreegDepartment of Molecular Biology and Biotechnology, College of Science, Sheffield University, Sheffield, TN, United Kingdom.
Kotb A AttiaRice Biotechnology Lab., Rice Research & Training Center (RRTC), Field Crops Research Institute, ARC, Sakhah, Egypt.ORCID 0000-0002-2992-1765
Stuart CassonDepartment of Molecular Biology and Biotechnology, College of Science, Sheffield University, Sheffield, TN, United Kingdom.
Sajid FiazDepartment of Plant Breeding and Genetics, The University of Haripur, Haripur, Pakistan.ORCID 0000-0001-9097-4359
Ebrahim A RamadanRice Biotechnology Lab., Rice Research & Training Center (RRTC), Field Crops Research Institute, ARC, Sakhah, Egypt.
Antar El- BannaDepartment of Genetics, College of Agriculture, Kafr-El-Sheikh University, Kafr El Sheikh, Egypt.
Nicholas ZouliasDepartment of Molecular Biology and Biotechnology, College of Science, Sheffield University, Sheffield, TN, United Kingdom.
Ali AboshoshaDepartment of Genetics, College of Agriculture, Kafr-El-Sheikh University, Kafr El Sheikh, Egypt.
Salman AlameryDepartment of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rice cultivation in Egypt is limited by the scarcity of water resources. The main strategy of rice breeders to overcome this problem is to develop new high-yielding varieties that are tolerant to drought stress. In this study, an drought-tolerant (IR60080-46A) variety was crossed with commercial Egyptian varieties using the back-cross method and marker-assisted selection (MAS) approach. The advanced lines of these crosses were selected under drought stress conditions. The best-performing candidate line, RBL-112, and its parental genotypes, were evaluated under drought stress and control conditions. The RBL-112 line showed superior its root system, which in turn produced higher grain yield under drought-stress conditions than its parental and check genotypes. Furthermore, physiological and biochemical studies showed that the RBL-112 line maintained higher relative water content (RWC), maximum quantum efficiency of photosystem II (Fv/Fm) values, proline content, superoxide dismutase (SOD) activity, and lower malondialdehyde (MDA) content compared to its parents and the check. The functional expression profiles of 22 drought tolerance-related genes were studied, out of which the genes OsAHL1, OsLEA3, OsCATA, OsP5CS, OsSNAC1, Os1g64660, OsRab21, OsAPX2, OsDREB2A, OsSKIPa, and OsLG3 were strongly induced in the newly developed RBL-112 line under drought-stress conditions. It could be concluded that the new line has a higher capacity to modulate physiological activities and expression levels of several drought-induced genes to withstand drought stress with high yielding ability. This finding suggests that the RBL-112 line presents a promising new addition to enable sustainable rice cultivation under water-limited conditions, and confirms the efficiency of the approach implemented in the current study.

Indexed as

DroughtsOryzaGenotypeStress, PhysiologicalWaterWater

Identifiers

PMID35349595
PMCPMC8963560

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.