ArticleEnvironmental science & technology2022
Detection of SARS-CoV-2 Proteins in Wastewater Samples by Mass Spectrometry.
Article in Environmental science & technology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Recent advances in MXene-molecularly imprinted polymer hybrid nanocomposites for electrochemical detection of pandemic-related biomarkers.Analytical and bioanalytical chemistry · 2026Review
- Impact of M and E protein mutations in SARS-CoV-2 Omicron variants on reduced antibody binding and increased structural stability: a bioinformatics study (2021-2023).BMC genomics · 2026Article
- From Gold to Polymer: Latex Bead-Assisted CRISPR-Cas12a Platform for Next-Generation Protein Diagnostics.ACS measurement science au · 2026Article
- Advances in Carbon Dot-Based Optical (Bio)Sensors for Contaminant Detection in Wastewater-Based Epidemiology.Sensors (Basel, Switzerland) · 2026Review
- Peptide Probe-Based NanoLC-MS/MS PRM for Ultrasensitive Detection of SARS-CoV-2 Proteins in Environmental Samples.Infectious diseases & clinical microbiology · 2026Article
- SARS-CoV-2 wastewater genomic surveillance: approaches, challenges, and opportunities.Genome biology · 2026Review
- Wastewater metaproteomics: tracking microbial and human protein biomarkers.ISME communications · 2026Article
- Immunoproteomics for wastewater-based health surveillance: A review.Environmental science and ecotechnology · 2025Review
- Advances in Wastewater-Based Epidemiology for Pandemic Surveillance: Methodological Frameworks and Future Perspectives.Microorganisms · 2025Review
- Article
- Research progress of severe acute respiratory syndrome coronavirus 2 on aerosol collection and detection.Chinese chemical letters = Zhongguo hua xue kuai bao · 2023Review
- Systematic scoping review evaluating the potential of wastewater-based epidemiology for monitoring cardiovascular disease and cancer.The Science of the total environment · 2023Article
- Advances in virus detection methods for wastewater-based epidemiological applications.Case studies in chemical and environmental engineering · 2022Article
- Recent Advances in CRISPR/Cas-Based Biosensors for Protein Detection.Bioengineering (Basel, Switzerland) · 2022Review
- SARS-CoV-2 Monitoring in Wastewater Reveals Novel Variants and Biomarkers of Infection.Viruses · 2022Article
- Understanding and managing uncertainty and variability for wastewater monitoring beyond the pandemic: Lessons learned from the United Kingdom national COVID-19 surveillance programmes.Journal of hazardous materials · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent COVID-19 pandemic overwhelmed the health system worldwide, and there was a need to track outbreaks and try to use this information as an early warning system. Wastewater-based epidemiology (WBE) enabled detection of the SARS-CoV-2 virus in wastewater treatment plant influents. Until now, the most used technique for this detection has been the quantitative polymerase chain reaction (qPCR)-based quantification of SARS-CoV-2 RNA. This study proposes a mass spectrometry (MS)-based method that detected specific SARS-CoV-2 proteins in wastewater, 5 and 6 days ahead of the case data for two municipalities. We identified unique peptides of eight proteins related to the SARS-CoV-2 virus and COVID-19 infection. We detected the nonstructural protein (NSP) pp1ab (transcribed after host cell infection) most frequently in all of the samples. As a result, we suspect that in the active cases of COVID-19, the pp1ab protein is present in high abundance in the urine and feces and that this protein could be used as an alternative biomarker. These data were collected before mass vaccination occurred in the population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.