Evidence map›Paper›PMID 35347788›Full record

ArticleIndoor air2022

Detection and isolation of airborne SARS-CoV-2 in a hospital setting.

Nuno Rufino de Sousa, Laura Steponaviciute, Lucille Margerie, Karolina Nissen, Midori Kjellin, Björn Reinius, Erik Salaneck, Klas I Udekwu, Antonio Gigliotti Rothfuchs

Erratum issuedAbstract read
In one paragraph

Article in Indoor air, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Nuno Rufino de SousaDepartment of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0002-0670-9788
Laura SteponaviciuteDepartment of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
Lucille MargerieDepartment of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0001-9537-1231
Karolina NissenDepartment of Medical Sciences, Infectious Diseases, Uppsala University, University Hospital Uppsala, Uppsala, Sweden.
Midori KjellinDepartment of Medical Sciences, Infectious Diseases, Uppsala University, University Hospital Uppsala, Uppsala, Sweden.ORCID 0000-0002-1377-6014
Björn ReiniusDepartment of Medical Biochemistry and Biophysics (MBB), Karolinska Institutet, Stockholm, Sweden.
Erik SalaneckDepartment of Medical Sciences, Infectious Diseases, Uppsala University, University Hospital Uppsala, Uppsala, Sweden.
Klas I UdekwuDepartment of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Antonio Gigliotti RothfuchsDepartment of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0001-6001-7240

Funding

Karolinska InstitutetScience for Life LaboratoryVetenskapsrådet
6 · The paper itself

Abstract

Transmission mechanisms for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are incompletely understood. In particular, aerosol transmission remains unclear, with viral detection in air and demonstration of its infection potential being actively investigated. To this end, we employed a novel electrostatic collector to sample air from rooms occupied by COVID-19 patients in a major Swedish hospital. Electrostatic air sampling in conjunction with extraction-free, reverse-transcriptase polymerase chain reaction (hid-RT-PCR) enabled detection of SARS-CoV-2 in air from patient rooms (9/22; 41%) and adjoining anterooms (10/22; 45%). Detection with hid-RT-PCR was concomitant with viral RNA presence on the surface of exhaust ventilation channels in patients and anterooms more than 2 m from the COVID-19 patient. Importantly, it was possible to detect active SARS-CoV-2 particles from room air, with a total of 496 plaque-forming units (PFUs) being isolated, establishing the presence of infectious, airborne SARS-CoV-2 in rooms occupied by COVID-19 patients. Our results support circulation of SARS-CoV-2 via aerosols and urge the revision of existing infection control frameworks to include airborne transmission.

Indexed as

Air Pollution, IndoorCOVID-19HospitalsHumansRNA, ViralSARS-CoV-2RNA, Viralenvironmental samplinghealth careinfectious aerosolspathogen detectionSARS-CoV-2transmission

Identifiers

PMID35347788
PMCPMC9111425

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.