ArticleNature methods2022
HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 64 citations in OpenAlex.
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- Chemical Biology 2025: Highlights From the Ch/Bi145 Course at Caltech.Chembiochem : a European journal of chemical biology · 2026Review
- Top-down regulation of ingestive behavior fragmentation.bioRxiv : the preprint server for biology · 2026Article
- 3D Visualization and Proteomic Analysis of Human Cardiac Transthyretin Amyloidosis Tissue Reveals Microangiopathy and Capillary Occlusion.bioRxiv : the preprint server for biology · 2026Article
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- A modular tissue-clearing framework integrated with light-field microscopy enables rapid volumetric phenotyping of cardiac tissue.Research square · 2026Article
- Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis.Journal of ophthalmology · 2026Review
- Multiscale 3D Whole Joint Cellular and Molecular Mapping Reveals Disease-Specific Neurovascular Plasticity Underlying the Structure-Pain Relationship.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- High-Throughput Volumetric Mapping Facilitated by Active Tissue SHRINK.Small methods · 2026Article
- PIEZO channels link mechanical forces to uterine contractions in parturition.Science (New York, N.Y.) · 2025Article
- Rapid sonication-assisted whole tissue clearing and immunostaining.Scientific reports · 2025Article
- Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- ADAPT-3D:accelerated deep adaptable processing of tissue for 3-dimensional fluorescence tissue imaging for research and clinical settings.Scientific reports · 2025Article
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- Generation of the Ci1 Reporter Mouse Strain with Enhanced Fluorescence for Tissue Clearing Applications.Neuroscience bulletin · 2025Article
- Spinal motor neuron development and metabolism are transcriptionally regulated by nuclear factor IA.Science advances · 2025Article
- Spiner, deep learning-based automated detection of spiral ganglion neurons in intact cochleae.iScience · 2025Article
- A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification.Communications biology · 2025Article
- Cartilage targeting cationic peptide carriers display deep cartilage penetration and retention in a rabbit model of post-traumatic osteoarthritis.Osteoarthritis and cartilage · 2025Article
- DNA-PAINT Imaging with Hydrogel Imprinting and Clearing.ACS sensors · 2025Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The recent development of solvent- and polymer-based brain-clearing techniques has advanced our ability to visualize the mammalian nervous system in three dimensions. However, it remains challenging to image the mammalian body en bloc. Here we developed HYBRiD (hydrogel-based reinforcement of three-dimensional imaging solvent-cleared organs (DISCO)), by recombining components of organic- and polymer-based clearing pipelines. We achieved high transparency and protein retention, as well as compatibility with direct fluorescent imaging and immunostaining in cleared mammalian bodies. Using parvalbumin- and somatostatin-Cre models, we demonstrated the utility of HYBRiD for whole-body imaging of genetically encoded fluorescent reporters without antibody enhancement of signals in newborn and juvenile mice. Using K18-hACE2 transgenic mice, HYBRiD enabled perfusion-free clearing and visualization of SARS-CoV-2 infection in a whole mouse chest, revealing macroscopic and microscopic features of viral pathology in the same sample. HYBRiD offers a simple and universal solution to visualize large heterogeneous body parts or entire animals for basic and translational research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.