ArticleNature biomedical engineering2022
High-speed light-sheet microscopy for the in-situ acquisition of volumetric histological images of living tissue.
Article in Nature biomedical engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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Who cites it
42 citing papers in PubMed, 75 citations in OpenAlex.
- Prototype-based AI triage for 3D pathology.bioRxiv : the preprint server for biology · 2026Article
- Dual-scale and dual-channel oblique plane microscopy for multi-resolution imaging of large tissue specimens.Biomedical optics express · 2026Article
- Chemical and Mechanical Regulation of Leukocyte Migration.Cold Spring Harbor perspectives in biology · 2026Review
- Single capture quantitative oblique back-illumination microscopy.Npj imaging · 2026Article
- High-Throughput Volumetric Mapping Facilitated by Active Tissue SHRINK.Small methods · 2026Article
- Deep UV laser scanning microscopy with integrated 1050 nm spectral-domain optical coherence tomography for multi-contrast tissue imaging.Biomedical optics express · 2025Article
- Multiplane 2.5D microscopy for high-throughput high-resolution tissue imaging.Journal of biomedical optics · 2025Article
- Fast and label-free 3D virtual H&E histology via active phase modulation-assisted dynamic full-field OCT.Npj imaging · 2025Article
- Tissue stiffness mapping by light sheet elastography.Science advances · 2025Article
- AI-based hardware and software tools in microscopy to boost research in immunology and virology.Frontiers in immunology · 2025Review
- Optical-resolution parallel ultraviolet photoacoustic microscopy for slide-free histology.Science advances · 2024Article
- Metabolic light absorption, scattering, and emission (MetaLASE) microscopy.Science advances · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- Mesoscale Brain Mapping: Bridging Scales and Modalities in Neuroimaging - A Symposium Review.Neuroinformatics · 2024Review
- Subdiffraction Imaging of Cleared and Expanded Large-Scale Tissues.Chemical & biomedical imaging · 2024Review
- Compact scattering-based light sheet microscopy probe using a custom miniature objective lens.Journal of optical microsystems · 2024Article
- Challenges of Spatially Resolved Metabolism in Cancer Research.Metabolites · 2024Review
- Article
- Super-resolution imaging of urethral vasculature in healthy pre- and post-menopausal females.iScience · 2024Article
- Article
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
Abstract
Histological examinations typically require the excision of tissue, followed by its fixation, slicing, staining, mounting and imaging, with timeframes ranging from minutes to days. This process may remove functional tissue, may miss abnormalities through under-sampling, prevents rapid decision-making, and increases costs. Here, we report the feasibility of microscopes based on swept confocally aligned planar excitation technology for the volumetric histological imaging of intact living tissue in real time. The systems' single-objective, light-sheet geometry and 3D imaging speeds enable roving image acquisition, which combined with 3D stitching permits the contiguous analysis of large tissue areas, as well as the dynamic assessment of tissue perfusion and function. Implemented in benchtop and miniaturized form factors, the microscopes also have high sensitivity, even for weak intrinsic fluorescence, allowing for the label-free imaging of diagnostically relevant histoarchitectural structures, as we show for pancreatic disease in living mice, for chronic kidney disease in fresh human kidney tissues, and for oral mucosa in a healthy volunteer. Miniaturized high-speed light-sheet microscopes for in-situ volumetric histological imaging may facilitate the point-of-care detection of diverse cellular-level biomarkers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.