Evidence map›Paper›PMID 35346271›Full record

ArticlePlant methods2022

Design and fabrication of an improved dynamic flow cuvette for

Sonia E Evans, Peter Duggan, Matthew E Bergman, Daniela Cobo-López, Benjamin Davis, Ibadat Bajwa, Michael A Phillips

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Sonia E EvansDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3G5, Canada.ORCID http://orcid.org/0000-0001-6775-1149
Peter DugganAcademic Machine Shop, University of Toronto-Mississauga, Mississauga, Canada.
Matthew E BergmanDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3G5, Canada.ORCID http://orcid.org/0000-0002-4071-3166
Daniela Cobo-LópezDepartment of Biology, University of Toronto-Mississauga, Mississauga, ON, L5L 1C6, Canada.
Benjamin DavisDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3G5, Canada.
Ibadat BajwaDepartment of Biology, University of Toronto-Mississauga, Mississauga, ON, L5L 1C6, Canada.
Michael A PhillipsDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3G5, Canada. michaelandrew.phillips@utoronto.ca.ORCID http://orcid.org/0000-0001-7276-119X
University of Toronto · CASt. Michael's Hospital · CA

Funding

Canadian Foundation for Innovation 36131Natural Sciences and Engineering Research Council of Canada RGPIN-2017-06400
6 · The paper itself

Abstract

backgroundStable isotope labeling is a non-invasive, sensitive means of monitoring metabolic flux in plants. The most physiologically meaningful information is obtained from experiments that take advantage of the natural photosynthetic carbon assimilation pathway to introduce a traceable marker with minimal effects on the physiology of the organism. The fundamental substrate in isotopic labeling experiments is

resultsHere we present a dynamic flow cuvette designed for single Arabidopsis thaliana labeling experiments. We have also verified its suitability for labeling Nicotiana benthamiana and essential oils in Pelargonium graveolens. Complete plans for fabrication of this device are included. The design includes three important innovations. First, uniform, circular air flow over the rosette surface is accomplished by a fan and deflector that creates a mini-cyclone effect within the chamber interior. Second, a network of circulating canals connected to a water bath provides temperature control to within ± 0.1 ºC under variable irradiance, humidity, and air flow conditions. When photosynthetically active radiation (PAR) was varied over a range of 1000 μEinsteins m

conclusionsAdvances in our ability to conduct short term labeling experiments are critical to understanding of the rates and control of the earliest steps in plant metabolism. Precise kinetic measurements in whole plants using

Indexed as

Carbon assimilationFlux analysisGas exchangeInstrumentationIsotopic labelingWhole plant physiology

Identifiers

PMID35346271
PMCPMC8958768
OpenAlexW4220850197

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.