ArticleBMC infectious diseases2022
Evaluation of five diagnostic methods for Strongyloides stercoralis infection in Amhara National Regional State, northwest Ethiopia.
Article in BMC infectious diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 3 of them syntheses that pooled it.
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20 citing papers in PubMed, 3 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Diagnostic accuracy of nucleic acid amplification tests for human intestinal nematode infections: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2026Pooled it
- Systematic Review of Strongyloides stercoralis Infection Diagnosis in Southeast Asia: Insights from Parasitological, Molecular, and Serological Approaches.The American journal of tropical medicine and hygiene · 2024Pooled it
- Prevalence of Strongyloides in Southeast Asia: a systematic review and meta-analysis with implications for public health and sustainable control strategies.Infectious diseases of poverty · 2023Pooled it
- Spotlighting zoonotic strongyloidiasis: a semi-systematic review of threadworms within baboons highlights opportunities for human infections.Infectious diseases of poverty · 2026Review
- Eosinophilia as a diagnostic window for strongyloidiasis: seroprevalence insights from an endemic region.BMC research notes · 2026Article
- Community survey of the infection, intensity and risk factors associated with schistosomiasis and soil-transmitted helminthiasis in south-eastern Gabon.Scientific reports · 2026Article
- Strongyloides steroralis: A case report to manifest the diagnosis and treatment.Caspian journal of internal medicine · 2026Article
- Review
- Factors associated with diagnostic delay and prognosis in hospitalized patients with strongyloidiasis in Okinawa, Japan.PLoS neglected tropical diseases · 2025Article
- Strongyloides stercoralis prevalence and diagnostic efficacy of an IgG4 rapid test in an eosinophilic population in Khuzestan Province, southwestern Iran.Parasites & vectors · 2025Article
- Surveillance of soil-transmitted helminths and other intestinal parasites in shelter dogs, Mississippi, USA.One health (Amsterdam, Netherlands) · 2025Article
- Detection of human strongyloidiasis among patients with a high risk of complications attending selected tertiary care hospitals in Colombo, Sri Lanka using molecular and serological diagnostic tools.Parasites & vectors · 2024Article
- The Recombinase Polymerase Amplification Test forPathogens (Basel, Switzerland) · 2024Article
- Comparison of parasitological methods for the identification of soil-transmitted helminths, includingParasite epidemiology and control · 2024Article
- Article
- First field study using Strong-LAMP for diagnosis of strongyloidiasis in Cubal, Angola.Parasites & vectors · 2023Article
- Intestinal helminth infections and associated risk factors among adults in the Lao People's Democratic Republic.Infectious diseases of poverty · 2023Article
- From past to present: opportunities and trends in the molecular detection and diagnosis of Strongyloides stercoralis.Parasites & vectors · 2023Review
- Prevalence ofDiagnostics (Basel, Switzerland) · 2023Article
- Advantages and Limitations of Microscopy and Molecular Detections for Diagnosis of Soil-transmitted Helminths: An Overview.Helminthologia · 2022Article
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9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStrongyloides stercoralis is an intestinal parasite that can cause chronic infection, hyperinfection and/or a dissemination syndrome in humans. The use of techniques targeting ova fails to detect S. stercoralis, as only larvae of the parasite are excreted in faeces. Due to the absence of "Gold" standard diagnostic method for S. stercoralis, there is a paucity of reported data worldwide.
objectiveThis study aimed to evaluate the performance of diagnostic methods of S. stercoralis infection by taking the composite reference as a "Gold" standard.
methodsA cross-sectional study was conducted among 844 schoolchildren in Amhara Region, Ethiopia, from April to December 2019. Stool samples were collected and processed with formol-ether concentration technique (FECT), spontaneous tube sedimentation technique (STST), Baermann concentration technique (BCT), agar plate culture (APC) and real-time polymerase chain reaction (RT-PCR). Sensitivity, specificity, positive predictive value, and negative predictive value of each diagnostic method were computed against the composite reference. The agreements of diagnostic methods were evaluated by Kappa value at 95% CI.
resultsThe composite detection rate of S. stercoralis by the five diagnostic methods was 39.0% (329/844). The detection rate of the parasite from stool samples by FECT, STST, BCT, APC and RT-PCR was 2.0% (17/844), 4.0% (34/844), 10.2% (86/844), 10.9% (92/844) and 28.8% (243/844), respectively. The highest detection rate (37.8%; 319/844) of S. stercoralis was recorded by a combination of BCT, APC, and RT-PCR followed by a combination of STST, BCT, APC and RT-PCR (37.3%; 315/844). The sensitivity of FECT, STST, BCT, APC and RT-PCR against the composite reference was 5.2%, 10.3%, 26.4%, 28.0% and 73.9%, respectively. The diagnostic agreements of RT-PCR, APC, BCT, STST and FECT with the composite reference in detection of S. stercoralis were substantial (0.775), fair (0.321), fair (0.305), slight (0.123), and slight (0.062), respectively.
conclusionRT-PCR detected the highest number of S. stercoralis infections. A combination of RT-PCR with APC and/or BCT better detected S. stercoralis from stool samples compared to other combinations or single diagnostic methods. Therefore, RT-PCR and combination of RT-PCR with APC and/or BCT diagnostic methods should be advocated for detection of S. stercoralis infection.
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