ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022
A Highly Sensitive CRISPR-Empowered Surface Plasmon Resonance Sensor for Diagnosis of Inherited Diseases with Femtomolar-Level Real-Time Quantification.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Biophysical characterization ofSensors & diagnostics · 2026Article
- Innovative Metasurface Colorimetric Cell-on-a-Chip Sensor for Continuous, Label-Free, Non-Destructive Assessment of Intestinal Barrier Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Investigating the molecular mechanisms of the "Tianma-Gouteng" herb pair in treating Parkinson's disease: a bioinformatics approach and density functional theory with molecular dynamics simulations validation.Frontiers in bioinformatics · 2026Article
- Appraisal of CRISPR Technology as an Innovative Screening to Therapeutic Toolkit for Genetic Disorders.Molecular biotechnology · 2026Review
- Recent Advances in Nanomedicine for Targeted Phototherapy in Head and Neck Cancer.International journal of nanomedicine · 2026Review
- A CRISPR-assisted passive microfluidic chip for rapid, visual detection of multiple respiratory viruses.Scientific reports · 2025Article
- 2D materials-based next-generation multidimensional photodetectors.Light, science & applications · 2025Review
- Self-Warning and Self-Repairing Mechanisms in Functional Coatings: A Review.Exploration (Beijing, China) · 2025Review
- Overcoming translational barriers in RNA-protein docking: enhancing computational accuracy for targeted drug discovery.Future medicinal chemistry · 2025Review
- Phototherapy in cancer treatment: strategies and challenges.Signal transduction and targeted therapy · 2025Review
- In Situ Printing of Polylactic Acid/Nanoceramic Filaments for the Repair of Bone Defects Using a Portable 3D Device.ACS applied materials & interfaces · 2025Review
- Graphdiyne biomaterials: from characterization to properties and applications.Journal of nanobiotechnology · 2025Review
- Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors.Biosensors · 2025Review
- Advancements in Glass Fiber Separator Technology for Lithium-Sulfur Batteries: The Role of Transport, Material Properties, and Modifications.ACS omega · 2025Review
- Advancements and challenges in brain cancer therapeutics.Exploration (Beijing, China) · 2024Review
- Free Electron Density Gradients Enhanced Biosensor for Ultrasensitive and Accurate Affinity Assessment of the Immunotherapy Drugs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Recent Advances in CRISPR/Cas System-Based Biosensors for the Detection of Foodborne Pathogenic Microorganisms.Micromachines · 2024Review
- CRISPR for companion diagnostics in low-resource settings.Lab on a chip · 2024Review
- Next-generation CRISPR/Cas-based ultrasensitive diagnostic tools: current progress and prospects.RSC advances · 2024Review
- Detection of biological loads in sewage using the automated robot-driven photoelectrochemical biosensing platform.Exploration (Beijing, China) · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system has emerged as a promising technology for the detection of nucleic acids. Herein, the development of a surface plasmon resonance (SPR) sensor that is functionalized with a layer of locally grown graphdiyne film, achieving excellent sensing performance when coupled with catalytically deactivated CRISPR-associated protein 9 (dCas9), is reported. dCas9 protein is immobilized on the sensor surface and complexed with a specific single-guide RNA, enabling the amplification-free detection of target sequences within genomic DNA. The sensor, termed CRISPR-SPR-Chip, is used to successfully analyze recombinant plasmids with only three-base mutations with a limit of detection as low as 1.3 fM. Real-time monitoring CRISPR-SPR-Chip is used to analyze clinical samples of patients with Duchenne muscular dystrophy with two exon deletions, which are detected without any pre-amplification step, yielding significantly positive results within 5 min. The ability of this novel CRISPR-empowered SPR (CRISPR-eSPR) sensing platform to rapidly, precisely, sensitively, and specifically detect a target gene sequence provides a new on-chip optic approach for clinical gene analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.