ArticleThe ISME journal2022
Meta-omics-aided isolation of an elusive anaerobic arsenic-methylating soil bacterium.
Article in The ISME journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- The key to arsenic pollution remediation: engineering and functional effects of arsenic-resistant microorganisms.World journal of microbiology & biotechnology · 2026Review
- Complex compositional and metabolic response of river sediment microbiomes to multiple anthropogenic stressors.ISME communications · 2026Article
- The balance between microbial arsenic methylation and demethylation in paddy soils underpins global arsenic risk and straighthead disease in rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Discovery and cultivation of prokaryotic taxa in the age of metagenomics and artificial intelligence.The ISME journal · 2025Article
- High-throughput cultivation and isolation of environmental anaerobes using selectively permeable hydrogel capsules.ISME communications · 2025Article
- Biosensor-aided isolation of anaerobic arsenic-methylating bacteria from soil.ISME communications · 2025Article
- Growth substrate limitation enhances anaerobic arsenic methylation byApplied and environmental microbiology · 2024Article
- Diversity and transcription of genes involved in respiratory As(V) reduction and As(III) methylation in Japanese paddy soils.BMC microbiology · 2024Article
- The arsenic bioremediation using genetically engineered microbial strains on aquatic environments: An updated overview.Heliyon · 2024Review
- Repression of microbial arsenite uptake and methylation by dissolved organic carbon.Environmental science & technology letters · 2024Article
- Controlling exposure to As and Cd from rice via irrigation management.Environmental geochemistry and health · 2024Article
- Soil Metaproteomics for Microbial Community Profiling: Methodologies and Challenges.Current microbiology · 2024Review
- Meta-omics assisted microbial gene and strain resources mining in contaminant environment.Engineering in life sciences · 2024Review
- Unraveling plant-microbe interactions: can integrated omics approaches offer concrete answers?Journal of experimental botany · 2024Article
- Soil redox status governs within-field spatial variation in microbial arsenic methylation and rice straighthead disease.The ISME journal · 2024Article
- Culturomics- and metagenomics-based insights into the soil microbiome preservation and application for sustainable agriculture.Frontiers in microbiology · 2024Review
- Arsenic and Microorganisms: Genes, Molecular Mechanisms, and Recent Advances in Microbial Arsenic Bioremediation.Microorganisms · 2023Review
- Methylotrophic methanogens and bacteria synergistically demethylate dimethylarsenate in paddy soil and alleviate rice straighthead disease.The ISME journal · 2023Article
- An overview of experimental simulations of microbial activity in early Earth.Frontiers in microbiology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Soil microbiomes harbour unparalleled functional and phylogenetic diversity. However, extracting isolates with a targeted function from complex microbiomes is not straightforward, particularly if the associated phenotype does not lend itself to high-throughput screening. Here, we tackle the methylation of arsenic (As) in anoxic soils. As methylation was proposed to be catalysed by sulfate-reducing bacteria. However, to date, there are no available anaerobic isolates capable of As methylation, whether sulfate-reducing or otherwise. The isolation of such a microorganism has been thwarted by the fact that the anaerobic bacteria harbouring a functional arsenite S-adenosylmethionine methyltransferase (ArsM) tested to date did not methylate As in pure culture. Additionally, fortuitous As methylation can result from the release of non-specific methyltransferases upon lysis. Thus, we combined metagenomics, metatranscriptomics, and metaproteomics to identify the microorganisms actively methylating As in anoxic soil-derived microbial cultures. Based on the metagenome-assembled genomes of microorganisms expressing ArsM, we isolated Paraclostridium sp. strain EML, which was confirmed to actively methylate As anaerobically. This work is an example of the application of meta-omics to the isolation of elusive microorganisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.