ArticleBMC medical genomics2022
MinION, a portable long-read sequencer, enables rapid vaginal microbiota analysis in a clinical setting.
Article in BMC medical genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A Monitoring Method to Evaluate the Accumulation of Antimicrobial-Resistance Genes in Gram-Negative Bacteria Distributed in Environmental Water.Environmental microbiology reports · 2025Article
- Profiling of vaginal microbial communities in Chilean women via self-sampling and nanopore sequencing.Scientific reports · 2025Article
- Four-Week Evaluation of the Interaction Pattern Among Saccharibacteria, Nitrate-Reducing Bacteria, and Periodontopathogens in Orthodontic Miniscrew Implants.Dentistry journal · 2025Article
- Characterization of vaginal microbiomes in clinician-collected bacterial vaginosis diagnosed samples.Microbiology spectrum · 2025Article
- Comparison of Illumina and Oxford Nanopore sequencing data quality for Clostridioides difficile genome analysis and their application for epidemiological surveillance.BMC genomics · 2025Article
- Methodological approaches in 16S sequencing of female reproductive tract in fertility patients: a review.Journal of assisted reproduction and genetics · 2025Review
- The application of long-read sequencing in clinical settings.Human genomics · 2023Review
- Rapid metagenomic sequencing for diagnosis and antimicrobial sensitivity prediction of canine bacterial infections.Microbial genomics · 2023Article
- Using nanopore sequencing to identify fungi from clinical samples with high phylogenetic resolution.Scientific reports · 2023Article
- Gut microbiome profiling of neonates using Nanopore MinION and Illumina MiSeq sequencing.Frontiers in microbiology · 2023Article
- Exploring taxonomic and functional microbiome of Hawaiian stream and spring irrigation water systems using Illumina and Oxford Nanopore sequencing platforms.Frontiers in microbiology · 2023Article
- Nanopore Sequencing Data Analysis of 16S rRNA Genes Using the GenomeSync-GSTK System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Improving analysis of the vaginal microbiota of women undergoing assisted reproduction using nanopore sequencing.Journal of assisted reproduction and genetics · 2022Article
- 16S rRNA nanopore sequencing for the diagnosis of ocular infection: a feasibility study.BMJ open ophthalmology · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt has been suggested that the local microbiota in the reproductive organs is relevant to women's health and may also affect pregnancy outcomes. Analysis of partial 16S ribosomal RNA (rRNA) gene sequences generated by short-read sequencers has been used to identify vaginal and endometrial microbiota, but it requires a long time to obtain the results, making it unsuitable for rapid bacterial identification from a small specimen amount in a clinical context.
methodsWe developed a simple workflow using the nanopore sequencer MinION that allows high-resolution and rapid differentiation of vaginal microbiota. Vaginal samples collected from 18 participants were subjected to DNA extraction and full-length 16S rRNA gene sequencing with MinION.
resultsThe principal coordinate analysis showed no differences in the bacterial compositions regardless of the sample collection method. The analysis of vaginal microbiota could be completed with a total analysis time of approximately four hours, allowing same-day results. Taxonomic profiling by MinION sequencing revealed relatively low diversity of the vaginal bacterial community, identifying the prevailing Lactobacillus species and several causative agents of bacterial vaginosis.
conclusionsFull-length 16S rRNA gene sequencing analysis with MinION provides a rapid means for identifying vaginal bacteria with higher resolution. Species-level profiling of human vaginal microbiota by MinION sequencing can allow the analysis of associations with conditions such as genital infections, endometritis, and threatened miscarriage.
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