Evidence map›Paper›PMID 35337270›Full record

ArticleBMC plant biology2022

De novo assembly of a fruit transcriptome set identifies AmMYB10 as a key regulator of anthocyanin biosynthesis in Aronia melanocarpa.

Jonathan D Mahoney, Sining Wang, Liam A Iorio, Jill L Wegrzyn, Matthew Dorris, Derek Martin, Bradley W Bolling, Mark H Brand, Huanzhong Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. 15-Foods (Basel, Switzerland) · 2024
    Article
  2. Investigating the induction of polyphenol biosynthesis in the culturedFrontiers in bioengineering and biotechnology · 2023
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jonathan D MahoneyDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Sining Wang *Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Liam A Iorio *Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Jill L WegrzynDepartment of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, 06269, USA.
Matthew DorrisDepartment of Food Science, University of Wisconsin, Madison, WI, 53706, USA.
Derek MartinDepartment of Food Science, University of Wisconsin, Madison, WI, 53706, USA.
Bradley W BollingDepartment of Food Science, University of Wisconsin, Madison, WI, 53706, USA.
Mark H BrandDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Huanzhong WangDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA. huanzhong.wang@uconn.edu.
University of Connecticut · USUniversity of Wisconsin–Madison · US

Funding

National Institute of Food and Agriculture 2017-67014-26157
6 · The paper itself

Abstract

Aronia is a group of deciduous fruiting shrubs, of the Rosaceae family, native to eastern North America. Interest in Aronia has increased because of the high levels of dietary antioxidants in Aronia fruits. Using Illumina RNA-seq transcriptome analysis, this study investigates the molecular mechanisms of polyphenol biosynthesis during Aronia fruit development. Six A. melanocarpa (diploid) accessions were collected at four fruit developmental stages. De novo assembly was performed with 341 million clean reads from 24 samples and assembled into 90,008 transcripts with an average length of 801 bp. The transcriptome had 96.1% complete according to Benchmarking Universal Single-Copy Orthologs (BUSCOs). The differentially expressed genes (DEGs) were identified in flavonoid biosynthetic and metabolic processes, pigment biosynthesis, carbohydrate metabolic processes, and polysaccharide metabolic processes based on significant Gene Ontology (GO) biological terms. The expression of ten anthocyanin biosynthetic genes showed significant up-regulation during fruit development according to the transcriptomic data, which was further confirmed using qRT-PCR expression analyses. Additionally, transcription factor genes were identified among the DEGs. Using a transient expression assay, we confirmed that AmMYB10 induces anthocyanin biosynthesis. The de novo transcriptome data provides a valuable resource for the understanding the molecular mechanisms of fruit anthocyanin biosynthesis in Aronia and species of the Rosaceae family.

Indexed as

PhotiniaTranscriptomeAnthocyaninsFruitGene Expression Regulation, PlantPlant ProteinsAnthocyaninsPlant ProteinsAnthocyaninAroniaRosaceaeTranscription factorTranscriptome

Identifiers

PMID35337270
PMCPMC8951710
OpenAlexW4221069763

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.