Evidence map›Paper›PMID 35331371›Full record

ArticleMethods in enzymology2022

Chemical proteomics for identifying short-chain fatty acid modified proteins in Salmonella.

Xinglin Yang, Zhenrun J Zhang, Howard C Hang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Xinglin YangDepartment of Immunology and Microbiology, Scripps Research, La Jolla, CA, United States.
Zhenrun J ZhangDuchossois Family Institute, The University of Chicago, Chicago, IL, United States.
Howard C HangDepartment of Immunology and Microbiology, Scripps Research, La Jolla, CA, United States; Department of Chemistry, Scripps Research, La Jolla, CA, United States. Electronic address: hhang@scripps.edu.
Scripps Institution of Oceanography · USScripps (United States) · USUniversity of Chicago · US

Funding

Studies on Protein LipidationR01GM087544 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI HANG, HOWARD C · 2010 to 2023
$4.4M
Distal gut microbiome targets of host anti-proteolytic proteins during colitisR21AI156674 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI HANG, HOWARD C · 2021 to 2022
$488k
NIAID NIH HHS R21 AI156674NIGMS NIH HHS R01 GM087544
6 · The paper itself

Abstract

Microbiota-metabolized small molecules play important roles to regulate host immunity and pathogen virulence. Specifically, microbiota generates millimolar concentration of short-chain fatty acid (SCFA) that can directly inhibit Salmonella virulence. Here, we describe chemical proteomic methods to identify SCFA-modified proteins in Salmonella using free fatty acids as well as their salicylic acid derivatives. In addition, we include CRISPR-Cas9 gene editing protocols for epitope-tagging of specific proteins to validate SCFA-modification in Salmonella. These protocols should facilitate the discovery and functional analysis of SCFA-modified proteins in Salmonella microbiology and pathogenesis.

Indexed as

MicrobiotaProteomicsFatty Acids, VolatileSalmonella typhimuriumFatty Acids, VolatileChemical proteomicsCRISPR-Cas9Epitope-taggingGut microbiota metabolitesSalmonella virulenceShort-chain fatty acidTarget identification

Identifiers

PMID35331371
PMCPMC9132015
OpenAlexW4220976305

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.