ArticleParasites & vectors2022
Saliva collection via capillary method may underestimate arboviral transmission by mosquitoes.
Article in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 57 citations in OpenAlex.
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- Variation in pathogen load and the pathogen load-infectiousness relationship broaden avian malaria's distribution.Nature communications · 2026Article
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- Contrasting La Crosse Virus Lineage III Vector Competency in Two Geographical Populations ofBiology · 2025Article
- Genotype-Specific Vector Competence ofViruses · 2025Article
- Heterogeneity in inhibition of genetically diverse dengue virus strains bybioRxiv : the preprint server for biology · 2025Article
- Evaluation of the Impact of Coinfection and Superinfection on Chikungunya and Mayaro Viruses' Replication inMicroorganisms · 2025Article
- Implications of successive blood feeding on Wolbachia-mediated dengue virus inhibition in Aedes aegypti mosquitoes.Nature communications · 2025Article
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- Mosquito immune cells enhance dengue and Zika virus infection in Aedes aegypti.Nature communications · 2025Article
- Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance.Insects · 2025Article
- InvasiveOne health (Amsterdam, Netherlands) · 2025Article
- Assessing Urban Yellow Fever Transmission Risk:Viruses · 2025Article
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- Investigating the Effects of Microclimate on Arboviral Kinetics inPathogens (Basel, Switzerland) · 2024Article
- Multiple bloodmeals enhance dissemination of arboviruses in three medically relevant mosquito genera.Parasites & vectors · 2024Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundArthropod-borne viruses (arboviruses) impose a major health and economic burden on human populations globally, with mosquitoes serving as important vectors. Measuring the ability of a mosquito population to transmit an arbovirus is important in terms of evaluating its public health risk. In the laboratory, a variety of methods are used to estimate arboviral transmission by mosquitoes, including indirect methods involving viral detection from mosquito saliva collected by forced salivation. The accuracy of indirect methods to estimate arbovirus transmission to live animal hosts has not been fully evaluated.
methodsWe compared three commonly used proxies of arboviral transmission, namely, the presence of virus in mosquito legs, in salivary glands (SG) and in saliva collected in capillary tubes using forced salivation, with direct transmission estimates from mosquitoes to suckling mice. We analyzed five vector-virus combinations, including Aedes aegypti infected with chikungunya virus, West Nile virus and Zika virus; Culex quinquefasciatus infected with West Nile virus; and Aedes triseriatus infected with La Crosse virus.
resultsComparatively, the methods of detecting virus infection in mosquito legs and in SG were equally accurate in predicting transmission. Overall, the presence of virus in mosquito legs was a more accurate predictor of transmission than the commonly implemented viral detection method using forced salivation into a capillary tube, and was subject to less technical variation.
conclusionsThese results suggest that, in general, forced salivation methods tend to underestimate virus transmission, and they provide confidence in the use of mosquito leg screens to evaluate the transmission potential of a mosquito population.
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