Evidence map›Paper›PMID 35331224›Full record

ArticleBMC biology2022

An extensional strain sensing mechanosome drives adhesion-independent platelet activation at supraphysiological hemodynamic gradients.

Nurul A Zainal Abidin, Eric K W Poon, Crispin Szydzik, Mariia Timofeeva, Farzan Akbaridoust, Rose J Brazilek, Francisco J Tovar Lopez, Xiao Ma, Chitrarth Lav, Ivan Marusic and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 19 citations in OpenAlex.

  1. Mechanisms of Piezo1-Mediated Mechanotransduction in Thrombotic Diseases.International journal of molecular sciences · 2026
    Review
  2. Article
  3. Selective Inhibition of Integrin β3 Topology Provides a Safer Antithrombotic Strategy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  4. Review
  5. Article
  6. Review
  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 2 countries.

Nurul A Zainal AbidinThe Australian Centre for Blood Diseases, Monash University, Melbourne, VIC, 3004, Australia.
Eric K W PoonDepartment of Medicine, St Vincent's Hospital, Melbourne Medical School, Faculty of Medicine, Dentistry & Health Sciences, The University of Melbourne, Fitzroy, VIC, 3065, Australia.
Crispin SzydzikThe Australian Centre for Blood Diseases, Monash University, Melbourne, VIC, 3004, Australia.
Mariia Timofeeva *Department of Mechanical Engineering, Faculty of Engineering and Information Technology, The University of Melbourne, Melbourne, VIC, 3010, Australia.
Farzan Akbaridoust *Department of Mechanical Engineering, Faculty of Engineering and Information Technology, The University of Melbourne, Melbourne, VIC, 3010, Australia.
Rose J BrazilekThe Australian Centre for Blood Diseases, Monash University, Melbourne, VIC, 3004, Australia.
Francisco J Tovar LopezSchool of Engineering, RMIT University, La Trobe Street, Melbourne, VIC, 3004, Australia.
Xiao MaMedicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, 3052, Australia.
Chitrarth LavDepartment of Mechanical Engineering, Faculty of Engineering and Information Technology, The University of Melbourne, Melbourne, VIC, 3010, Australia.
Ivan MarusicDepartment of Mechanical Engineering, Faculty of Engineering and Information Technology, The University of Melbourne, Melbourne, VIC, 3010, Australia.
Philip E ThompsonMedicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, 3052, Australia.
Arnan MitchellSchool of Engineering, RMIT University, La Trobe Street, Melbourne, VIC, 3004, Australia.
Andrew S H OoiDepartment of Mechanical Engineering, Faculty of Engineering and Information Technology, The University of Melbourne, Melbourne, VIC, 3010, Australia.
Justin R HamiltonThe Australian Centre for Blood Diseases, Monash University, Melbourne, VIC, 3004, Australia.
Warwick S NesbittThe Australian Centre for Blood Diseases, Monash University, Melbourne, VIC, 3004, Australia. warwick.nesbitt@monash.edu.ORCID 0000-0001-5644-7053
The University of Melbourne · AUAustralian Centre for HIV and Hepatitis Virology Research · AUMonash University · AURMIT University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSupraphysiological hemodynamics are a recognized driver of platelet activation and thrombosis at high-grade stenosis and in blood contacting circulatory support devices. However, whether platelets mechano-sense hemodynamic parameters directly in free flow (in the absence of adhesion receptor engagement), the specific hemodynamic parameters at play, the precise timing of activation, and the signaling mechanism(s) involved remain poorly elucidated.

resultsUsing a generalized Newtonian computational model in combination with microfluidic models of flow acceleration and quasi-homogenous extensional strain, we demonstrate that platelets directly mechano-sense acute changes in free-flow extensional strain independent of shear strain, platelet amplification loops, von Willebrand factor, and canonical adhesion receptor engagement. We define an extensional strain sensing "mechanosome" in platelets involving cooperative Ca

conclusionsOur findings suggest that platelets are adapted to rapidly respond to supraphysiological extensional strain dynamics, rather than the peak magnitude of imposed wall shear stress. In the context of overall platelet activation and thrombosis, we posit that "extensional strain sensing" acts as a priming mechanism in response to threshold levels of extensional strain allowing platelets to form downstream adhesive interactions more rapidly under the limiting effects of supraphysiological hemodynamics.

Indexed as

Platelet ActivationThrombosisBlood PlateletsHemodynamicsHumansIon ChannelsStress, Mechanicalvon Willebrand FactorIon ChannelsPIEZO1 protein, humanvon Willebrand FactorExtensional strainHemodynamicsMechanotransductionPlatelet

Identifiers

PMID35331224
PMCPMC8944166
OpenAlexW4220942183

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.