ArticleEuropean journal of histochemistry : EJH2022
Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity.
Article in European journal of histochemistry : EJH, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
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- Loss of the Ubiquitin-Associated Domain of sqstm1/p62 in Zebrafish Causes a Phenotype Resembling Paget's Disease of Bone.Calcified tissue international · 2025Article
- FTO-associated osteoclastogenesis promotes alveolar bone resorption in apical periodontitis male rat via the HK1/USP14/RANK pathway.Nature communications · 2025Article
- Breast cancer cells promote osteoclast differentiation in an MRTF-dependent paracrine manner.Molecular biology of the cell · 2025Article
- Reprogramming macrophages with R848-loaded artificial protocells to modulate skin and skeletal wound healing.Journal of cell science · 2024Article
- Neutrophils in Inflammatory Bone Diseases.Current osteoporosis reports · 2024Review
- Biological Activities of Deer Antler-Derived Peptides on Human Chondrocyte and Bone Metabolism.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Osteomodulin downregulation is associated with osteoarthritis development.Bone research · 2023Article
- Transcriptome Profiling of Osteoblasts in a Medaka (Frontiers in cell and developmental biology · 2022Article
- Real-time quantification of soluble tartrate-resistant acid phosphatase as a measure of osteoclastogenesis.BioTechniquesArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histochemical detection of tartrate-resistant acid phosphatase (TRAP) activity is a fundamental technique for visualizing osteoclastic bone resorption and assessing osteoclast activity status in tissues. This approach has mostly employed colorimetric detection, which has limited quantification of activity in situ and co-labelling with other skeletal markers. Here we report simple colorimetric and fluorescent TRAP assays in zebrafish and medaka, two important model organisms for investigating the pathogenesis of bone disorders. We show fluorescent TRAP staining, utilising the ELF97 substrate, is a rapid, robust and stable system to visualise and quantify osteoclast activity in zebrafish, and is compatible with other fluorescence stains, transgenic lines and antibody approaches. Using this approach, we show that TRAP activity is predominantly found around the base of the zebrafish pharyngeal teeth, where osteoclast activity state appears to be heterogeneous.
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