ArticleInternational journal of molecular sciences2022
Effects of Cryopreservation on Sperm with Cryodiluent in Viviparous Black Rockfish (
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- High-Efficiency Cryopreservation of Silver Pomfret Sperm: Protocol Development and Cryodamage Assessment.Animals : an open access journal from MDPI · 2025Article
- Precursor A-Kinase Anchor Protein 4 as a Predictive Biomarker of Post-Thaw Semen Quality in Goats.Veterinary sciences · 2025Article
- The role of ladderlectin in spermatogenesis and ovarian sperm storage in the black rockfish (Sebastes schlegelii).Communications biology · 2025Article
- Cryopreservation Protocol Optimization forBiology · 2025Article
- Chloroplast DNA methylation in the kelpEvolutionary applications · 2024Article
- Comparative transcriptome analysis reveals molecular damage associated with cryopreservation in Crassostrea angulata D-larvae rather than to cryoprotectant exposure.BMC genomics · 2024Article
- Population Genomics of Commercial FishGenes · 2024Article
- Novel Apoplastic Antifreeze Proteins ofBiomolecules · 2024Article
- The residue of salinomycin in the muscles of olive flounder (Paralichthys olivaceus) and black rockfish (Sebastes Schlegeli) after oral administration analyzed by LC-Tandem-MS.BMC veterinary research · 2024Article
- Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa.Scientific reports · 2023Article
- Establishment of aBiology · 2023Article
- High temperature influences DNA methylation and transcriptional profiles in sea urchins (Strongylocentrotus intermedius).BMC genomics · 2023Article
- Cryopreservation of Roughscale Sole (Animals : an open access journal from MDPI · 2022Article
- Evaluation ofAnimals : an open access journal from MDPI · 2022Article
- Transcriptome Analysis Reveals Key Gene Expression Changes in Blue Catfish Sperm in Response to Cryopreservation.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Black rockfish is an economically important fish in East Asia. Little mention has been paid to the sperm cryopreservation in black rockfish. In this study, the optimal cryodiluent was selected from 48 combinations by detecting various sperm parameters. Transcriptome and methylome analysis were further performed to explore the molecular mechanism of inevitable cryoinjuries. The results showed that cryopreservation had negative effects on the viability, DNA integrity, mitochondrial activity, total ATPase and LDH of sperm even with optimal cryodiluent (FBS + 15% Gly). Transcriptome and methylome analysis revealed that the expression of 179 genes and methylation of 1266 genes were affected by cryopreservation. These genes were enriched in GO terms of death, G-protein coupled receptor signaling pathway, response to external stimulus and KEGG pathways of phospholipase D signaling pathway and xenobiotic and carbohydrate metabolism pathways. The role of PIK3CA and CCNA2 were highlighted in the protein-protein interaction network, and the sperm quality-related imprinted gene
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