Evidence map›Paper›PMID 35328696›Full record

ArticleInternational journal of molecular sciences2022

Chitosan Hydrogel Supplemented with Metformin Promotes Neuron-like Cell Differentiation of Gingival Mesenchymal Stem Cells.

Shanglin Cai, Tong Lei, Wangyu Bi, Shutao Sun, Shiwen Deng, Xiaoshuang Zhang, Yanjie Yang, Zhuangzhuang Xiao, Hongwu Du

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 29 citations in OpenAlex.

  1. Review
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  7. Proteomics and Metabolomics in Biomedicine.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Shanglin CaiSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Tong LeiSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Wangyu BiSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Shutao SunInstitutional Center for Shared Technologies and Facilities, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Shiwen DengSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.ORCID 0000-0002-9082-4876
Xiaoshuang ZhangSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Yanjie YangSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Zhuangzhuang XiaoSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Hongwu DuSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
University of Science and Technology Beijing · CNChinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human gingival mesenchymal stem cells (GMSCs) are derived from migratory neural crest stem cells and have the potential to differentiate into neurons. Metformin can inhibit stem-cell aging and promotes the regeneration and development of neurons. In this study, we investigated the potential of metformin as an enhancer on neuronal differentiation of GMSCs in the growth environment of chitosan hydrogel. The crosslinked chitosan/β-glycerophosphate hydrogel can form a perforated microporous structure that is suitable for cell growth and channels to transport water and macromolecules. GMSCs have powerful osteogenic, adipogenic and chondrogenic abilities in the induction medium supplemented with metformin. After induction in an induction medium supplemented with metformin, Western blot and immunofluorescence results showed that GMSCs differentiated into neuron-like cells with a significantly enhanced expression of neuro-related markers, including Nestin (NES) and β-Tubulin (TUJ1). Proteomics was used to construct protein profiles in neural differentiation, and the results showed that chitosan hydrogels containing metformin promoted the upregulation of neural regeneration-related proteins, including ATP5F1, ATP5J, NADH dehydrogenase (ubiquinone) Fe-S protein 3 (NDUFS3), and Glutamate Dehydrogenase 1 (GLUD1). Our results help to promote the clinical application of stem-cell neural regeneration.

Indexed as

ChitosanMesenchymal Stem CellsMetforminCell DifferentiationCells, CulturedGingivaHumansHydrogelsNeuronsChitosanHydrogelsMetforminchitosan hydrogelgingival mesenchymal stem cellmetforminneuronal differentiation

Identifiers

PMID35328696
PMCPMC8955038
OpenAlexW4220787545

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.