Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I.

Helen Bergquist, Raviteja Inturi, Rula Zain, Tanel Punga

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Helen BergquistDepartment of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, Husargatan 3, SE-751 23 Uppsala, Sweden.

Raviteja InturiDepartment of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, Husargatan 3, SE-751 23 Uppsala, Sweden.ORCID 0000-0003-1344-3962

Rula ZainDepartment of Laboratory Medicine, Translational Research Center Karolinska (TRACK), Karolinska Institutet, Huddinge, SE-141 86 Stockholm, Sweden.

Tanel PungaDepartment of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, Husargatan 3, SE-751 23 Uppsala, Sweden.ORCID 0000-0002-0561-367X

Funding

Ländells Foundation 2016Marcus Borgströms Foundation 2016Swedish Cancer Society 2014Swedish Research Council 2015

6 · The paper itself

Abstract

RNA molecules can adopt specific RNA triplex structures to execute critical biological functions. Human adenoviruses (HAdVs) are abundant pathogens encoding the essential, noncoding virus-associated RNA I (VA RNAI). Here, we employ a triplex-specific probing assay, based on the intercalating and cleaving agent benzoquinoquinoxaline 1, 10-phenanthroline (BQQ-OP), to unravel a potential RNA triplex formation in VA RNAI. The BQQ-OP cleavage of the pathogenic HAdV type 4 (HAdV-4) VA RNAI indicates that a potential triplex is formed involving the highly conserved stem 4 of the central domain and side stem 7. Further, the integrity of the HAdV-4 VA RNAI side stem 7 contributes to a potential triplex formation in vitro and virus growth in vivo. Collectively, we propose that the HAdV-4 VA RNAI can potentially form a biologically relevant triplex structure.

Indexed as

Adenoviruses, HumanHumansRNA, BacterialRNA, BacterialRNA IadenovirusBQQ–OPtriplex RNAVA RNAI

Identifiers

PMID35328524

DOI10.3390/ijms23063103

PMCPMC8949993

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.