ArticleInternational journal of molecular sciences2022
Cryoprotectants-Free Vitrification and Conventional Freezing of Human Spermatozoa: A Comparative Transcript Profiling.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Cryopreservation of Human Spermatozoa Using Penetrating Cryoprotectant-Free Vitrification Medium Exhibits an Altered Proteomic Profile Compared to the Conventional Rapid Freezing Method.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Beyond swimming: emerging parameters for predicting the fertility of mouse spermatozoa.Lab animal · 2025Review
- Successful Cryoprotectant-Free Vitrification of Honey Bee (Apis mellifera) Drone Sperm With Royal Jelly Supplemented Extender.Veterinary medicine and science · 2025Article
- The role of heat shock protein 70 on oocyte apoptosis during vitrification.Open veterinary journal · 2025Review
- The storage time of cryopreserved human spermatozoa does not affect pathways involved in fertility.Basic and clinical andrology · 2024Article
- A Pilot Analysis of Whole Transcriptome of Human Cryopreserved Sperm.International journal of molecular sciences · 2024Article
- Comparative Transcriptomic Analyses for the Optimization of Thawing Regimes during Conventional Cryopreservation of Mature and Immature Human Testicular Tissue.International journal of molecular sciences · 2023Article
- Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects.International journal of molecular sciences · 2023Review
- Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability.International journal of molecular sciences · 2023Review
- What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution.Journal of clinical medicine · 2023Review
- Integrated transcriptomics and proteomics assay identifies the role of FCGR1A in maintaining sperm fertilization capacity during semen cryopreservation in sheep.Frontiers in cell and developmental biology · 2023Article
- Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation.Animal reproduction · 2023Review
- Epigenetic Alterations in Cryopreserved Human Spermatozoa: Suspected Potential Functional Defects.Cells · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionSpermatozoa cryopreservation is an important technique to preserve fertility for males. This study aimed at exploring the stability of epigenetics information in human spermatozoa, manipulated by two different technologies, freezing and vitrification.
methodsSpermatozoa samples were distributed into three groups: 1. Fresh spermatozoa (control group), 2. Frozen spermatozoa, 3. Vitrified spermatozoa. Epigenetic differences of fresh and cryopreserved spermatozoa were evaluated using high-throughput RNA sequencing.
resultsDifferentially expressed genes (DEGs) in frozen (1103 genes) and vitrified (333 genes) spermatozoa were evaluated. The bioinformatical analysis identified 8 and 15 significant pathways in groups of frozen and vitrified spermatozoa, respectively. The majority of these pathways are most relevant to immune and infectious diseases. The DEGs of the fertilization process are not detected during vitrification. The freezing process induces more down-regulation of genes and is relevant to apoptosis changes and immune response.
conclusionCryopreservation of human spermatozoa is an epigenetically safe method for male fertility preservation. Cryoprotectant-free vitrification can induce more minor biological changes in human spermatozoa, in comparison with conventional freezing.
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