Evidence map›Paper›PMID 35327670›Full record

ArticleBiomolecules2022

Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation.

Yan Liang, Qisong Gao, Haiyang Wang, Mengling Guo, Abdelaziz Adam Idriss Arbab, Mudasir Nazar, Mingxun Li, Zhangping Yang, Niel A Karrow, Yongjiang Mao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Yan LiangKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0001-9747-4357
Qisong GaoKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Haiyang WangKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Mengling GuoKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Abdelaziz Adam Idriss ArbabKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0002-8783-2859
Mudasir NazarKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Mingxun LiKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0002-0323-254X
Zhangping YangKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0002-5479-8447
Niel A KarrowCenter for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Yongjiang MaoKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Department of Animal Breeding and Production, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Yangzhou University · CNUniversity of Guelph · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, circular RNAs (circRNAs) from Holstein cow mammary tissues were identified and compared between early lactation and non-lactation. After analysis, 10,684 circRNAs were identified, ranging from 48 to 99,406 bp, and the average size was 882 bp. The circRNAs were mainly distributed on chromosomes 1 to 11, and 89.89% of the circRNAs belonged to sense-overlapping circRNA. The exons contained with circRNAs ranged from 1 to 47 and were concentrated from 1 to 5. Compared with the non-lactating cows, 87 circRNAs were significantly differentially expressed in the peak lactation cows. There were 68 upregulated circRNAs and 19 downregulated circRNAs. Enrichment analysis of circRNAs showed that GO analysis mainly focused on immune response, triglyceride transport, T cell receptor signaling pathway, etc. Pathway analysis mainly focused on cytokine-cytokine receptor interaction, T helper 17 cell differentiation, fatty acid biosynthesis, the JAK-STAT signaling pathway, etc. Specific primers were designed for two proximal ends of the circRNA junction sites to allow for PCR validation of four randomly selected circRNAs and carry out circRNA-miRNA interaction research. This study revealed the expression profile and characteristics of circRNAs in mammary tissue from Holstein cows at early lactation and non-lactation, thus providing rich information for the study of circRNA functions and mechanisms, as well as potential candidate miRNA genes for studying lactation in Holstein cows.

Indexed as

MicroRNAsRNA, CircularAnimalsCattleFemaleGene Expression ProfilingLactationMicroRNAsRNA, Circularcircular RNAHolstein cowsmammary tissueRNA sequencing

Identifiers

PMID35327670
PMCPMC8946036
OpenAlexW4220760790

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.