Evidence map›Paper›PMID 35326418›Full record

ArticleCells2022

3D In Vitro Platform for Cell and Explant Culture in Liquid-like Solids.

Duy T Nguyen, Jack E Famiglietti, Ryan A Smolchek, Zadia Dupee, Nickolas Diodati, Diego I Pedro, Juan M Urueña, Matthew A Schaller, W Gregory Sawyer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
  6. Article
  7. 3D Culture Analysis of Cancer Cell Adherence toTissue engineering. Part C, Methods · 2024
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Duy T NguyenDepartment of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.
Jack E FamigliettiDepartment of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.ORCID 0000-0002-1429-0534
Ryan A SmolchekDepartment of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.
Zadia DupeeDivision of Pulmonary, Critical Care, and Sleep Medicine, University of Florida, Gainesville, FL 32611, USA.
Nickolas DiodatiDepartment of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.
Diego I PedroDepartment of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.
Juan M UrueñaDepartment of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.
Matthew A SchallerDivision of Pulmonary, Critical Care, and Sleep Medicine, University of Florida, Gainesville, FL 32611, USA.
W Gregory SawyerDepartment of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.

Funding

National Science Foundation DGE-1842473
6 · The paper itself

Abstract

Existing 3D cell models and technologies have offered tools to elevate cell culture to a more physiologically relevant dimension. One mechanism to maintain cells cultured in 3D is by means of perfusion. However, existing perfusion technologies for cell culture require complex electronic components, intricate tubing networks, or specific laboratory protocols for each application. We have developed a cell culture platform that simply employs a pump-free suction device to enable controlled perfusion of cell culture media through a bed of granular microgels and removal of cell-secreted metabolic waste. We demonstrated the versatile application of the platform by culturing single cells and keeping tissue microexplants viable for an extended period. The human cardiomyocyte AC16 cell line cultured in our platform revealed rapid cellular spheroid formation after 48 h and ~90% viability by day 7. Notably, we were able to culture gut microexplants for more than 2 weeks as demonstrated by immunofluorescent viability assay and prolonged contractility.

Indexed as

Cell Culture TechniquesSpheroids, CellularCell LineHumansPerfusion3D cell culturein vitroliquid-like solidsmicroexplantsmicrogelsperfusion

Identifiers

PMID35326418
PMCPMC8946834

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.