ArticleMethods in molecular biology (Clifton, N.J.)2022
Fine-Tuning Plant Gene Expression with Synthetic Trans-Acting Small Interfering RNAs.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Effective Gene Silencing in Plants by Synthetic Trans-Acting siRNAs Derived From Minimal Precursors.Bio-protocol · 2025Article
- Syn-tasiR-VIGS: virus-based targeted RNAi in plants by synthetic trans-acting small interfering RNAs derived from minimal precursors.Nucleic acids research · 2025Article
- Combining AI and new genomic techniques to 'fine-tune' plants: challenges in risk assessment.Frontiers in plant science · 2025Article
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Authors and funding
4 authors.
Funding
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Abstract
RNAi-based tools are widely used in gene function studies and for crop improvement. However, no effective methods for precisely controlling the degree of induced silencing have been reported until recently. Here we report a detailed protocol for designing and generating synthetic trans-acting small interfering RNA (syn-tasiRNA) constructs for fine-tuning gene expression in plants. Recently developed high-throughput AtTAS1c-D2-B/c-based vectors are used to clone and express syn-tasiRNAs that possess different efficacies depending on their precursor location and on their degree of base-pairing with the 5' end of target RNAs.
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Registered trials
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