Evidence map›Paper›PMID 35325421›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Optimization of Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing (VIGS) in Tomato.

Ashish Kumar Singh, Dibyendu Ghosh, Supriya Chakraborty

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Functional identification of DNA demethylase geneFrontiers in plant science · 2024
    Article
  5. Plants (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ashish Kumar SinghMolecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.ORCID 0000-0001-9619-6252
Dibyendu GhoshMolecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.ORCID 0000-0001-9707-0635
Supriya ChakrabortyMolecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. supriyachakrasls@yahoo.com.ORCID 0000-0001-9301-2649

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Unveiling of full genome sequence of tomato demands significant advances of tomato functional genomics. Virus-induced gene silencing (VIGS) is a well explored functional genomics tool in plant biology that exploits post transcriptional gene silencing to downregulate a desired gene. Although VIGS provides an easy and highly efficient platform to study plant gene function through reverse genetics approach, currently VIGS is more efficient in model plants like Nicotiana benthamiana, which further justifies the urgent need of a highly efficient, reliable, and reproducible VIGS protocol in crop plants such as tomato. In this chapter, we have detailed an optimized Tobacco rattle virus (TRV)-based VIGS protocol in tomato.

Indexed as

NicotianaSolanum lycopersicumGene SilencingGenetic VectorsPlant VirusesAgrobacteriumFunctional genomicsPDSPTGSTomatoTRVVIGS

Identifiers

PMID35325421

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.