ArticleMethods in molecular biology (Clifton, N.J.)2022
Barley Stripe Mosaic Virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes in Wheat and Barley.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions.Genetics and molecular biology · 2026Article
- Genome-Wide Identification and Analysis of theInternational journal of molecular sciences · 2023Article
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2 authors.
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Abstract
Virus-induced gene silencing (VIGS) is an efficient method for functional characterization of genes in monocot and dicot plants via transient silencing of gene(s) of interest. Among various virus vectors, Barley stripe mosaic virus (BSMV) is established as a vector of choice to silence genes in wheat and barley. BSMV is a single-stranded positive-sense RNA virus with a tripartite genome consisting of α, β, and γ RNAs. BSMV-based VIGS has been used to silence both abiotic and biotic stress response genes in various growth stages of plants. Here we describe an efficient and effective protocol to successfully silence wheat and barley genes expressing in various tissues using this approach.
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