Evidence map›Paper›PMID 35325180›Full record

ArticleMicroscopy (Oxford, England)2022

Correlative volume-imaging using combined array tomography and FIB-SEM tomography with beam deceleration for 3D architecture visualization in tissue.

Shingo Hirashima, Keisuke Ohta, Yukiko Rikimaru-Nishi, Akinobu Togo, Takashi Funatsu, Risa Tsuneyoshi, Yuichi Shima, Kei-Ichiro Nakamura

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microscopy (Oxford, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shingo HirashimaDepartment of Anatomy, Division of Microscopic and Developmental Anatomy, Kurume University School of Medicine, Kurume 830-0011, Japan.ORCID 0000-0002-6558-6482
Keisuke OhtaDepartment of Anatomy, Division of Microscopic and Developmental Anatomy, Kurume University School of Medicine, Kurume 830-0011, Japan.
Yukiko Rikimaru-NishiDepartment of Anatomy, Division of Microscopic and Developmental Anatomy, Kurume University School of Medicine, Kurume 830-0011, Japan.
Akinobu TogoAdvanced Imaging Research Center, Kurume University School of Medicine, Kurume 830-0011, Japan.
Takashi FunatsuAdvanced Imaging Research Center, Kurume University School of Medicine, Kurume 830-0011, Japan.
Risa TsuneyoshiDepartment of Anatomy, Division of Microscopic and Developmental Anatomy, Kurume University School of Medicine, Kurume 830-0011, Japan.
Yuichi ShimaDepartment of Anatomy, Division of Microscopic and Developmental Anatomy, Kurume University School of Medicine, Kurume 830-0011, Japan.
Kei-Ichiro NakamuraDepartment of Anatomy, Division of Microscopic and Developmental Anatomy, Kurume University School of Medicine, Kurume 830-0011, Japan.ORCID 0000-0003-3438-9176

Funding

Japan Society for the Promotion of Science Grant-in-Aid for Early-Career Scientists [grant nuJapan Society for the Promotion of Science Grant-in-Aid for Scientific Research (B) [grant nKazato Research Foundation Kazato Research Encourage Prize 2020
6 · The paper itself

Abstract

Focused ion beamed (FIB) SEM has a higher spatial resolution than other volume-imaging methods owing to the use of ion beams. However, in this method, it is challenging to analyse entire biological structures buried deep in the resin block. We developed a novel volume-imaging method by combining array tomography and FIB-SEM tomography and investigated the chondrocyte ultrastructure. Our method imparts certainty in determining the analysis area such that cracks or areas with poor staining within the block are avoided. The chondrocyte surface showed fine dendritic processes that were thinner than ultrathin sections. Upon combination with immunostaining, this method holds promise for analysing mesoscopic architectures.

Indexed as

DecelerationTomographyImaging, Three-DimensionalMicroscopy, Electron, ScanningTomography, X-Ray Computedarray tomographyFIB-SEMmesoscopic architectureserial block-face scanning electron microscopythree-dimensional analysisvolume imaging

Identifiers

PMID35325180
PMCPMC9169539

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.