ArticleFEMS microbiology letters2022
Cultivation of ammonia-oxidising archaea on solid medium.
Article in FEMS microbiology letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Cultivation and molecular profiling reveal ammonia-oxidizing archaea as skin commensals.The ISME journal · 2026Article
- Environmental distribution, physiology and genomic adaptations of arctic ammonia-oxidizing archaea.Frontiers in microbiology · 2026Article
- Overcoming gelling agent-derived inhibitory factors enables the cultivation of fastidious nitrifiers on solid media.ISME communications · 2026Article
- Viral infection collapses intracytoplasmic membrane integrity and autotrophic metabolism in ammonia-oxidizingISME communications · 2026Article
- Genomic profiling of soil nitrifying microorganisms enriched on floating membrane filter.Journal of microbiology (Seoul, Korea) · 2025Article
- Isolation and cultivation of a novel freshwater magnetotactic coccus FCR-1 containing unchained magnetosomes.Communications biology · 2025Article
- Distinct Patterns of Antibiotic Sensitivities in Ammonia-Oxidising Archaea.Environmental microbiology · 2025Article
- Biofilm lifestyle across different lineages of ammonia-oxidizing archaea.The ISME journal · 2025Article
- Obtaining complete and canonical ammonia-oxidizing bacteria through specific labeling and cell sorting.ISME communications · 2025Article
- The Archaeal Cell Cycle.Annual review of cell and developmental biology · 2024Review
- Activity of novel virus families infecting soil nitrifiers is concomitant with host niche differentiation.The ISME journal · 2024Article
- Activity-based labelling of ammonia- and alkane-oxidizing microorganisms including ammonia-oxidizing archaea.ISME communications · 2024Article
- Characterisation of bacteria representing a novel Nitrosomonas clade: Physiology, genomics and distribution of missing ammonia oxidizer.Environmental microbiology reports · 2023Article
- A Simple and Effective Method for Solid Medium Cultivation of Strictly Hydrogen- and Sulfur-oxidizing Chemolithoautotrophs Predominant in Deep-sea Hydrothermal Fields.Microbes and environments · 2023Article
- Shedding light on the composition of extreme microbial dark matter: alternative approaches for culturing extremophiles.Frontiers in microbiology · 2023Review
- Discovery of Oleaginous Yeast from Mountain Forest Soil in Thailand.Journal of fungi (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Ammonia-oxidising archaea (AOA) are environmentally important microorganisms involved in the biogeochemical cycling of nitrogen. Routine cultivation of AOA is exclusively performed in liquid cultures and reports on their growth on solid medium are scarce. The ability to grow AOA on solid medium would be beneficial for not only the purification of enrichment cultures but also for developing genetic tools. The aim of this study was to develop a reliable method for growing individual colonies from AOA cultures on solid medium. Three phylogenetically distinct AOA strains were tested: 'Candidatus Nitrosocosmicus franklandus C13', Nitrososphaera viennensis EN76 and 'Candidatus Nitrosotalea sinensis Nd2'. Of the gelling agents tested, agar and Bacto-agar severely inhibited growth of all three strains. In contrast, both 'Ca. N. franklandus C13' and N. viennensis EN76 tolerated Phytagel™ while the acidophilic 'Ca. N. sinensis Nd2' was completely inhibited. Based on these observations, we developed a Liquid-Solid (LS) method that involves immobilising cells in Phytagel™ and overlaying with liquid medium. This approach resulted in the development of visible distinct colonies from 'Ca. N. franklandus C13' and N. viennensis EN76 cultures and lays the groundwork for the genetic manipulation of this group of microorganisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.