Evidence map›Paper›PMID 35322853›Full record

ArticleJournal of cell science2022

Restoration of DNA integrity and the cell cycle by electric stimulation in planarian tissues damaged by ionizing radiation.

Devon Davidian, Melanie LeGro, Paul G Barghouth, Salvador Rojas, Benjamin Ziman, Eli Isael Maciel, David Ardell, Ariel L Escobar, Néstor J Oviedo

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Devon DavidianDepartment of Molecular & Cell Biology, University of California, Merced, CA 95343, USA.
Melanie LeGroDepartment of Molecular & Cell Biology, University of California, Merced, CA 95343, USA.
Paul G BarghouthDepartment of Molecular & Cell Biology, University of California, Merced, CA 95343, USA.
Salvador RojasDepartment of Molecular & Cell Biology, University of California, Merced, CA 95343, USA.
Benjamin ZimanDepartment of Molecular & Cell Biology, University of California, Merced, CA 95343, USA.
Eli Isael MacielDepartment of Molecular & Cell Biology, University of California, Merced, CA 95343, USA.
David ArdellDepartment of Molecular & Cell Biology, University of California, Merced, CA 95343, USA.ORCID 0000-0001-7313-0963
Ariel L EscobarHealth Sciences Research Institute, University of California, Merced, CA 95343, USA.
Néstor J OviedoDepartment of Molecular & Cell Biology, University of California, Merced, CA 95343, USA.ORCID 0000-0002-0213-9781
University of California, Merced · US

Funding

The Effects of Direct Current Stimulation in Adult TissuesR01GM132753 · NIGMS · UNIVERSITY OF CALIFORNIA, MERCED · PI OVIEDO, NESTOR J · 2019 to 2022
$1.2M
Acquisition of Covaris E220 and Sciclone G3 systems for high throughput sequencinS10OD010786 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI COMAI, LUCA · 2012 to 2012
$311k
National Institutes of Health National Institute of General Medical Sciences R01GM132753National Science Foundation 1744620NIGMS NIH HHS R01 GM132753NIH HHS S10 OD010786University of California Cancer Research Coordinating Committee CRR-18-525108
6 · The paper itself

Abstract

Exposure to high levels of ionizing γ radiation leads to irreversible DNA damage and cell death. Here, we establish that exogenous application of electric stimulation enables cellular plasticity and the re-establishment of stem cell activity in tissues damaged by ionizing radiation. We show that subthreshold direct current stimulation (DCS) rapidly restores pluripotent stem cell populations previously eliminated by lethally γ-irradiated tissues of the planarian flatworm Schmidtea mediterranea. Our findings reveal that DCS enhances DNA repair, transcriptional activity, and cell cycle entry in post-mitotic cells. These responses involve rapid increases in cytosolic Ca2+ concentration through the activation of L-type Cav channels and intracellular Ca2+ stores, leading to the activation of immediate early genes and ectopic expression of stem cell markers in post-mitotic cells. Overall, we show the potential of electric current stimulation to reverse the damaging effects of high-dose γ radiation in adult tissues. Furthermore, our results provide mechanistic insights describing how electric stimulation effectively translates into molecular responses capable of regulating fundamental cellular functions without the need for genetic or pharmacological intervention.

Indexed as

PlanariansAnimalsCalciumCell CycleDNAElectric StimulationRadiation, IonizingCalciumDNADNA repairElectric stimulationGalvanotacticNeoblastsPlanariaStem cellsTissue regeneration

Identifiers

PMID35322853
PMCPMC9264365
OpenAlexW4221090176

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.