ArticleScientific reports2022
CRISPR-mediated knockout of cardinal and cinnabar eye pigmentation genes in the western tarnished plant bug.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- RNAi-Mediated Knockdown of Halloween Genes in Lygus hesperus Has Varied Effects on Oogenesis and Fertility.Archives of insect biochemistry and physiology · 2026Article
- The life history of recessive deleterious alleles as seen through the eyes of a honey bee (Apis mellifera).Molecular biology and evolution · 2026Article
- Eye can see clearly now: Identifying the locus associated with a white-eye mutation in honey bees (Apis mellifera).Insect molecular biology · 2026Article
- Inheritance Mode of a Red-Eye Mutation inInsects · 2025Article
- SYNCAS based CRISPR-Cas9 gene editing in predatory mites, whiteflies and stinkbugs.Insect biochemistry and molecular biology · 2025Article
- Pioneering genome editing in parthenogenetic stick insects: CRISPR/Cas9-mediated gene knockout in Medauroidea extradentata.Scientific reports · 2025Article
- Adapting and Testing ReMOT Control for Expanded CRISPR-Era Genome Functions in Non-model Insects.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Manipulation of a New Non-model Insect Genome Using Targeted CRISPR-Era Approaches.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Gene editing in agricultural, health, and veterinary pest arthropods: recent advances.Current opinion in insect science · 2024Review
- Genome editing of the vermilion locus generates a visible eye color marker for Oncopeltus fasciatus.Scientific reports · 2023Article
- Article
- Gene Editing and Genetic Control of Hemipteran Pests: Progress, Challenges and Perspectives.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The western tarnished plant bug, Lygus hesperus, is a key hemipteran pest of numerous agricultural, horticultural, and industrial crops in the western United States and Mexico. A lack of genetic tools in L. hesperus hinders progress in functional genomics and in developing innovative pest control methods such as gene drive. Here, using RNA interference (RNAi) against cardinal (LhCd), cinnabar (LhCn), and white (LhW), we showed that knockdown of LhW was lethal to developing embryos, while knockdown of LhCd or LhCn produced bright red eye phenotypes, in contrast to wild-type brown eyes. We further used CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated) genome editing to generate germline knockouts of both LhCd (Card) and LhCn (Cinn), producing separate strains of L. hesperus characterized by mutant eye phenotypes. Although the cardinal knockout strain Card exhibited a gradual darkening of the eyes to brown typical of the wild-type line later in nymphal development, we observed bright red eyes throughout all life stages in the cinnabar knockout strain Cinn, making it a viable marker for tracking gene editing in L. hesperus. These results provide evidence that CRISPR/Cas9 gene editing functions in L. hesperus and that eye pigmentation genes are useful for tracking the successful genetic manipulation of this insect.
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