Evidence map›Paper›PMID 35320683›Full record

ReviewAnnual review of biochemistry2022

Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.

Eugene Y D Chua, Joshua H Mendez, Micah Rapp, Serban L Ilca, Yong Zi Tan, Kashyap Maruthi, Huihui Kuang, Christina M Zimanyi, Anchi Cheng, Edward T Eng and 3 more

Open access · hybridAbstract readReview
In one paragraph

Review in Annual review of biochemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.

0numbers the graph read from it
0cells of the map it votes in
91citing papers in PubMed
30.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

91 citing papers in PubMed, 156 citations in OpenAlex.

  1. eLife · 2026
    Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Optimized bacterial expression of a synthetic BRIL antibody.Acta crystallographica. Section F, Structural biology communications · 2026
    Article
  7. Article
  8. Determinants of metal import and specificity in a bacterial transporter.bioRxiv : the preprint server for biology · 2026
    Article
  9. Structural Basis for CbioRxiv : the preprint server for biology · 2026
    Article
  10. Article
  11. Article
  12. Review
  13. CryoAtom improves model building for cryo-EM.Nature structural & molecular biology · 2026
    Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. The amazing AAV capsids: Into the structure-verse.Molecular therapy. Methods & clinical development · 2025
    Review
  19. Review
  20. Review

31 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Eugene Y D ChuaNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Joshua H MendezNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Micah RappNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Serban L IlcaNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Yong Zi TanDepartment of Biological Sciences, National University of Singapore, Singapore; email: yztan@nus.edu.sg.
Kashyap MaruthiNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Huihui KuangNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Christina M ZimanyiNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Anchi ChengNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Edward T EngNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Alex J NobleNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Clinton S PotterNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
Bridget CarragherNew York Structural Biology Center, New York, NY, USA; email: echua@nysbc.org, jmendez@nysbc.org, mrapp@nysbc.org, silca@nysbc.org, kmaruthi@nysbc.org, hkuang@nysbc.org, czimanyi@nysbc.org, acheng@nysbc.org, eeng@nysbc.org, anoble@nysbc.org, cpotter@nysbc.org, bcarr@nysbc.org.
New York Structural Biology Center · USSimons Foundation · USNational University of Singapore · SG

Funding

NCCAT: National Center for CryoEM Access and Training- Supplement for Windows 10 and FFIU24GM129539 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2018 to 2023
$53.9M
TRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2012 to 2021
$14.7M
The National Center for In-situ Tomographic Ultramicroscopy (NCITU) SupplementU24GM139171 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2020 to 2025
$8.7M
NIGMS NIH HHS P41 GM103310NIGMS NIH HHS U24 GM129539NIGMS NIH HHS U24 GM139171
6 · The paper itself

Abstract

Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.

Indexed as

COVID-19PandemicsCryoelectron MicroscopyHumansSARS-CoV-2Single Molecule Imagingautomationcryo-EMdemocratizationin situ tomographymachine learningSARS-CoV-2

Identifiers

PMID35320683
PMCPMC10393189
OpenAlexW4220916482

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.