Evidence map›Paper›PMID 35320674›Full record

ArticleACS synthetic biology2022

Charge Engineering Improves the Performance of Bst DNA Polymerase Fusions.

Inyup Paik, Sanchita Bhadra, Andrew D Ellington

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 33 citations in OpenAlex.

  1. Review
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  14. Article
  15. Bst polymerase - a humble relative of Taq polymerase.Computational and structural biotechnology journal · 2023
    Review
  16. Loop-Mediated Isothermal Amplification: From Theory to Practice.Russian journal of bioorganic chemistry · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Inyup PaikDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0001-5938-6380
Sanchita BhadraDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0002-4956-2405
Andrew D EllingtonDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0001-6246-5338
The University of Texas at Austin · US

Funding

Directed evolution of polymerases that can read and write extremely long sequencesR01EB027202 · NIBIB · UNIVERSITY OF TEXAS AT AUSTIN · PI ELLINGTON, ANDREW D · 2020 to 2024
$1.6M
NIBIB NIH HHS R01 EB027202
6 · The paper itself

Abstract

The charge states of proteins can greatly influence their stabilities and interactions with substrates, and the addition of multiple charges (supercharging) has been shown to be a successful approach for engineering protein stability and function. The addition of a fast-folding fusion domain to the

Indexed as

DNA-Directed DNA PolymeraseNucleic Acid Amplification TechniquesDNA ReplicationProtein EngineeringRNA-Directed DNA PolymeraseSensitivity and SpecificityDNA-Directed DNA PolymeraseRNA-Directed DNA PolymeraseBr512charge engineeringfusion domainshigh-temperature LAMPpolymerase engineeringsupercharging

Identifiers

PMID35320674
PMCPMC9514385
OpenAlexW4220946384

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.