ArticleDevelopment (Cambridge, England)2022
In vitro culture of ovine embryos up to early gastrulating stages.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular Regulation of Post-Hatching Embryo Development: Implications for Early Pregnancy Losses in Livestock Species.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Interleukin-6 and leukemia inhibitory factor differentially modify embryonic disk composition in post hatching bovine embryos†.Biology of reproduction · 2026Article
- Development and characterization of functional sheep endometrial luminal epithelial organoids.Veterinary research · 2026Article
- Bovine uterine fluid lipidome parallels embryo and conceptus developmental milestones†.Biology of reproduction · 2026Article
- Modelling mammalian implantationAnimal reproduction · 2026Review
- Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The guinea pig serves as an alternative model to study human preimplantation development.Nature cell biology · 2025Article
- Primed bovine embryonic stem cell lines can be derived at diverse stages of blastocyst development with similar efficiency and molecular characteristics.Biology open · 2025Article
- SMC2 ablation impairs bovine embryo development shortly after blastocyst hatching.Reproduction (Cambridge, England) · 2024Article
- Interleukin-6 supplementation improves bovine conceptus elongation and transcriptomic indicators of developmental competence†.Biology of reproduction · 2024Article
- Pig blastocyst-like structure models from embryonic stem cells.Cell discovery · 2024Article
- Understanding bovine embryo elongation: a transcriptomic study of trophoblastic vesicles.Frontiers in physiology · 2024Article
- Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle.Animals : an open access journal from MDPI · 2023Review
- Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developmental failures occurring shortly after blastocyst hatching from the zona pellucida constitute a major cause of pregnancy losses in both humans and farm ungulates. The developmental events occurring following hatching in ungulates include the proliferation and maturation of extra-embryonic membranes - trophoblast and hypoblast - and the formation of a flat embryonic disc, similar to that found in humans, which initiates gastrulation prior to implantation. Unfortunately, our understanding of these key processes for embryo survival is limited because current culture systems cannot sustain ungulate embryo development beyond hatching. Here, we report a culture system that recapitulates most developmental landmarks of gastrulating ovine embryos: trophoblast maturation, hypoblast migration, embryonic disc formation, disappearance of the Rauber's layer, epiblast polarization and mesoderm differentiation. Our system represents a highly valuable platform for exploring the cell differentiation, proliferation and migration processes governing gastrulation in a flat embryonic disc and for understanding pregnancy failures during the second week of gestation. This article has an associated 'The people behind the papers' interview.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.