Evidence map›Paper›PMID 35317874›Full record

ArticleThe Journal of laryngology and otology2023

Establishment of four head and neck squamous cell carcinoma cell lines: importance of reference DNA for accurate genomic characterisation.

K B Patel, S Prokopec, J W Barrett, J S Mymryk, P C Boutros, A C Nichols

Abstract read
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In one paragraph

Article in The Journal of laryngology and otology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

K B PatelDepartment of Head and Neck - Endocrine Oncology, Moffit Cancer Center, Tampa, Florida, USA.ORCID https://orcid.org/0000-0002-2285-0386
S ProkopecInformatics and Biocomputing Platform Ontario Institute for Cancer Research, Toronto, Canada.
J W BarrettDepartment of Otolaryngology - Head and Neck Surgery, Western University, London, Ontario, Canada.
J S MymrykDepartment of Otolaryngology - Head and Neck Surgery, Western University, London, Ontario, Canada.
P C BoutrosDepartment of Human Genetics, University of California, Los Angeles, USA.
A C NicholsDepartment of Otolaryngology - Head and Neck Surgery, Western University, London, Ontario, Canada.

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
CIHR MOP 377832NCI NIH HHS P30 CA016042NIH/NCI
6 · The paper itself

Abstract

objectiveThere is significant interest in developing early passage cell lines with matched normal reference DNA to facilitate a precision medicine approach in assessing drug response. This study aimed to establish early passage cell lines, and perform whole exome sequencing and short tandem repeat profiling on matched normal reference DNA, primary tumour and corresponding cell lines.

methodsA cell culture based,

resultsFour early passage cell lines were established. Two cell lines were human papillomavirus positive, confirmed by sequencing and p16 immunoblotting. Short tandem repeat profiling confirmed that all cell lines were established from their index tumours. Whole exome sequencing revealed that the matched normal reference DNA was critical for accurate mutational analysis: a high rate of false positive mutation calls were excluded (87.6 per cent).

conclusionEarly passage cell lines were successfully established. Patient-matched reference DNA is important for accurate cell line mutational calls.

Indexed as

Carcinoma, Squamous CellHead and Neck NeoplasmsPapillomavirus InfectionsCell LineCyclin-Dependent Kinase Inhibitor p16DNA, ViralGenomicsHumansSquamous Cell Carcinoma of Head and NeckCyclin-Dependent Kinase Inhibitor p16DNA, ViralCell LineHead And Neck Squamous Cell CarcinomaHPVHPV-Positive Cell LinesHuman PapillomavirusMutation

Identifiers

PMID35317874
PMCPMC9975763

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.