Evidence map›Paper›PMID 35316608›Full record

ReviewAnnual review of analytical chemistry (Palo Alto, Calif.)2022

Label-Free Super-Resolution Imaging Techniques.

Ryan E Leighton, Ariel M Alperstein, Renee R Frontiera

Abstract readReview
In one paragraph

Review in Annual review of analytical chemistry (Palo Alto, Calif.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Mixed Domain IR-Hyper-Raman Four Wave Mixing Spectroscopy.The journal of physical chemistry letters · 2026
    Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ryan E LeightonDepartment of Chemistry, University of Minnesota, Minneapolis, Minnesota, USA; email: rrf@umn.edu.
Ariel M AlpersteinDepartment of Chemistry, University of Minnesota, Minneapolis, Minnesota, USA; email: rrf@umn.edu.
Renee R FrontieraDepartment of Chemistry, University of Minnesota, Minneapolis, Minnesota, USA; email: rrf@umn.edu.

Funding

Label-Free Super-Resolution Imaging of Membrane ProteinsR35GM119441 · NIGMS · UNIVERSITY OF MINNESOTA · PI FRONTIERA, RENEE R · 2016 to 2020
$1.4M
NIGMS NIH HHS R35 GM119441
6 · The paper itself

Abstract

Biological and material samples contain nanoscale heterogeneities that are unresolvable with conventional microscopy techniques. Super-resolution fluorescence methods can break the optical diffraction limit to observe these features, but they require samples to be fluorescently labeled. Over the past decade, progress has been made toward developing super-resolution techniques that do not require the use of labels. These label-free techniques span a variety of different approaches, including structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies. Many draw inspiration from widely successful fluorescence-based techniques such as stimulated emission depletion (STED) microscopy, photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). In this review, we discuss the progress made in these fields along with the current challenges and prospects in reaching resolutions comparable to those achieved with fluorescence-based methods.

Indexed as

Microscopy, Fluorescencechemical imagingcoherent Raman imaginglabel-freephotothermal infraredsubdiffractionsuper-resolution Raman microscopy

Identifiers

PMID35316608
PMCPMC9454238

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.